|
| Status |
Public on Apr 04, 2019 |
| Title |
control/PortoTorres(PT) 4 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
pooled samples of digestive gland tissue
|
| Organism |
Mytilus galloprovincialis |
| Characteristics |
treatment: caged for 30 days in Formelli (control)
gender: female
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted from the digestive gland of a single individual mussel using Trizol following manufacturer's instructions
|
| Label |
Cy3
|
| Label protocol |
5 µg of total RNA were primed with 0.5 µg oligodT(19)VN primer at 70°C for 10 min, then reversed transcribed at 42°C for 2 h in the presence of 400 U ReverseAid MuLV H minus RTase (Fermentas), and 100 µM each dATP, dTTP, dGTP, with 25 µM dCTP, 25 µM Cy3-labeled dCTP or Cy5-label dCTP
|
| |
|
| Channel 2 |
| Source name |
pooled samples of digestive gland tissue
|
| Organism |
Mytilus galloprovincialis |
| Characteristics |
treatment: caged for 30 days in Porto Tores
gender: female
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted from the digestive gland of a single individual mussel using Trizol following manufacturer's instructions
|
| Label |
Cy5
|
| Label protocol |
5 µg of total RNA were primed with 0.5 µg oligodT(19)VN primer at 70°C for 10 min, then reversed transcribed at 42°C for 2 h in the presence of 400 U ReverseAid MuLV H minus RTase (Fermentas), and 100 µM each dATP, dTTP, dGTP, with 25 µM dCTP, 25 µM Cy3-labeled dCTP or Cy5-label dCTP
|
| |
|
| |
| Hybridization protocol |
43°C, 16/20 h, hybridization buffer Northern Max (Ambion). Pre-hybridization for 1h in same conditions.
|
| Scan protocol |
Scanned on Chip Reader (Applied biosystems Laboratories) at 5 µm resolution
|
| Data processing |
Offset background subtracted, global mean normalisation across microarray surfaces and local mean normalisation across element signal intensity. Data obtained from log2 of processed Ch2 signal / processed Ch1 signal.
|
| |
|
| Submission date |
Mar 27, 2017 |
| Last update date |
Apr 04, 2019 |
| Contact name |
Gerolamo Lanfranchi |
| E-mail(s) |
stefano.cagnin@unipd.it
|
| Phone |
+39-0498276219
|
| Organization name |
University of Padova
|
| Department |
CRIBI - Biotechnology Center and Biology Department
|
| Lab |
Functional Genomics Lab
|
| Street address |
Via U. Bassi, 58/B
|
| City |
Padova |
| ZIP/Postal code |
35131 |
| Country |
Italy |
| |
|
| Platform ID |
GPL22172 |
| Series (1) |
| GSE97099 |
Field application of a new low density stress response microarray STREM |
|
