|
| Status |
Public on Sep 14, 2017 |
| Title |
Primary AML patient LAM33 |
| Sample type |
RNA |
| |
|
| Source name |
pediatric t(8;21)RUNX1-RUNX1T1 AML BM
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Bone Marrow
aberration: t(8;21)RUNX1-RUNX1T1
dataset: Training-set
age class: >10
ckit mutation: WT
Sex: M
aberration: 10000-100000
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total bone marrow RNA was extracted using TRIzol reagent (Invitrogen) according to manufacturer guidelines, quantified using UV spectroscopy (Nanodrop Technologies), and qualitatively assessed using an Agilent2100 Bioanalyzer (Agilent Technologies).
|
| Label |
biotin
|
| Label protocol |
Samples were labeled using the Affymetrix FlashTag™ Biotin HSR RNA Labeling Kit as per manufacturer's protocols.
|
| |
|
| Hybridization protocol |
The GeneChip® Hybridization, Wash, and Stain Kit was used as per manufacturer’s protocols. The GeneChip® Fluidics Station 450 was used for washing and staining with fluidics script FS450_0002.
|
| Scan protocol |
Microarray chips were read on the GeneChip® Scanner 3000 7G with AGCC version 4.0.0.1567.
|
| Description |
miRNA expression data from pediatric t(8;21)AML patient harboring FLT3-ITD mutation (gene-level)
|
| Data processing |
The CEL files generated were analyzed through Affymetrix Expression Console Software (version 1.3) which normalizes array signals using a robust multiarray averaging (RMA) algorithm.
|
| |
|
| Submission date |
Mar 28, 2017 |
| Last update date |
Sep 14, 2017 |
| Contact name |
Matteo Zampini |
| E-mail(s) |
m.zampini@irpcds.org
|
| Phone |
+390498211466
|
| Organization name |
Istituto di Ricerca Pediatrica (IRP) Città della Speranza
|
| Street address |
corso stati uniti 4
|
| City |
Padova |
| State/province |
PD |
| ZIP/Postal code |
35127 |
| Country |
Italy |
| |
|
| Platform ID |
GPL21572 |
| Series (1) |
| GSE97135 |
A miRNA-based signature associated to relapse in pediatric t(8;21)RUNX1-RUNX1T1 |
|
