|
| Status |
Public on Mar 31, 2017 |
| Title |
Denver_006 |
| Sample type |
RNA |
| |
|
| Source name |
preoptic area/hypothalamus/pituitary
|
| Organism |
Xenopus laevis |
| Characteristics |
treatment: recombinant X. laevis leptin (rxLeptin; 20 ng/g BW)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA extraction with Trizol followed by Qiagen total RNA extraction columns
|
| Label |
biotin
|
| Label protocol |
Biotinylated aRNA were prepared according to the Affymetrix GeneChip 3’ IVT Express kit protocol from 250 ng total RNA (Affymetrix GeneChip 3’ IVT Express kit Instruction Manual, P/N 702646 Rev. 5)
|
| |
|
| Hybridization protocol |
Following fragmentation, 16 ug of aRNA were hybridized for 16 hr at 45C on Xenopus Genome 2.0 GeneChips. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix 3000 7G GeneChip Scanner with Autoloader.
|
| Data processing |
Data was analyzed using the limma and affy Bioconductor packages implemented in the R-statistical environment. RMA was used to normalize the data and fit log2 transformed expression values.
|
| |
|
| Submission date |
Mar 30, 2017 |
| Last update date |
Mar 31, 2017 |
| Contact name |
Robert J Denver |
| E-mail(s) |
rdenver@umich.edu
|
| Phone |
7349366625
|
| Organization name |
University of Michigan
|
| Department |
MCDB
|
| Street address |
1105 North University Ave
|
| City |
Ann Arbor |
| State/province |
Michigan |
| ZIP/Postal code |
48109-1085 |
| Country |
USA |
| |
|
| Platform ID |
GPL10756 |
| Series (1) |
| GSE97243 |
Acute (2 hr) response to rxLeptin injection in preoptic area/hypothalamus/pituitary of early prometamorphic (NF stage 54) Xenopus laevis tadpoles |
|
