|
| Status |
Public on Apr 01, 2017 |
| Title |
C14 human term placenta from AGA pregnancy |
| Sample type |
RNA |
| |
|
| Source name |
whole human placenta isolated from the fetal aspect
|
| Organism |
Homo sapiens |
| Characteristics |
age: 39 wks
gender: F newborn
delivery: C/section
birth weight: 3460g
|
| Extracted molecule |
total RNA |
| Extraction protocol |
total miRNA from human placentas was purified using the Qiagen miRNeasy Mini Kit (Qiagen, Valencia, CA) per manufacturer's protocol. Tissue was prepared by adding 700uL QIAzol lysis reagent to 50 mg of placental tissue and homogenized for one minute using the Omni tissue homogenizer. After elution of the miRNA, the Qiagen RNeasy MinElute Cleanup Kit was used to remove contaminating DNA and to concentrate the samples. RNA concentration and quality were measured using NonoDrop 2000c spectrophotometer (ThermoFisher Scientific).
|
| Label |
biotin
|
| Label protocol |
The RNA was labeled using the FlashTag Biotin HSR RNA labeling Kit (Affymetrix), per the manufacturer's protocol starting with 8ug of RNA. Briefly, starting with total RNA, the process involved a brief tailing reaction followed by ligation of the biotinylated signal molecule to the target RNA sample.
|
| |
|
| Hybridization protocol |
Hybridization was performed per the manufacturer's protocol (Affymetrix). Briefly, the hybridization cocktail mix is prepared as per the appropriate starting volume, denature the hybridization cocktail and centrifuge with cocktail and transfer into the appropriate well of the hybridization tray.
|
| Scan protocol |
The scanner used is the GeneChip Scanner 3000 7G, scanner type M10, software version Express console 1.4.1.46
|
| Description |
miRNA expression data from whole term human placenta
|
| Data processing |
Microarray analysis was performed by the Clinical Microarray Core facility at UCLA, using the Affymetrix GeneChip miRNA 4.0 Array. The data obtained was initially analyzed using the Express Console Software version 1.4.1.46 (Affymetrix).
|
| |
|
| Submission date |
Mar 31, 2017 |
| Last update date |
Apr 01, 2017 |
| Contact name |
Alison Chu |
| E-mail(s) |
AlisonChu@mednet.ucla.edu
|
| Organization name |
UCLA
|
| Street address |
10833 LeConte Avenue
|
| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90095 |
| Country |
USA |
| |
|
| Platform ID |
GPL21572 |
| Series (1) |
| GSE97294 |
miRNA data in human placentas from normal pregnancies and from pregnancies complicated by SGA and IUGR [miRNA-4] |
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