|
| Status |
Public on Jun 04, 2018 |
| Title |
30d_WT_rep2_head |
| Sample type |
SRA |
| |
|
| Source name |
fly head
|
| Organism |
Drosophila melanogaster |
| Characteristics |
tissue: head
age: 30 day
genotype: 5905
|
| Treatment protocol |
Fly heads were harvested at the given age.
|
| Growth protocol |
Flies were cultured in standard media at 25ºC with 60% humidity unless otherwise specified. The control (WT) line used was 5905 (FlyBase ID FBst0005905, w1118). The given genotype male flies were collected at the day of eclosion and transferred to new vials every other day during aging process.
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
For RNA-Seq, RNA was isolated by TRIZol reagent, and polyA tail RNAs were selected.
RNA libraries were prepared according to instructions of NEB Ultra RNA library prep kit (NEB, E7530L).
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
NextSeq 550 |
| |
|
| Description |
processed data file: WT_vs_Pcl_Suz12_head_DESeq.csv
|
| Data processing |
For RNA-Seq comparison, Sequencing reads were mapped to dm6 with STAR by default parameter. The read counts for each genes were calculated by HTSeq-count. The count files were used as input to DESeq for differential expression analysis.
Genome_build: dm6
Supplementary_files_format_and_content: DESeq was used for calling differential expression. .CSV files contain the DESeq output.
|
| |
|
| Submission date |
Mar 31, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Hui Wang |
| E-mail(s) |
wanghuisimm@sioc.ac.cn, mazj@sioc.ac.cn
|
| Organization name |
Chinese Academy of Science
|
| Street address |
Qiuyue Road 26
|
| City |
Shanghai |
| ZIP/Postal code |
200032 |
| Country |
China |
| |
|
| Platform ID |
GPL22106 |
| Series (1) |
| GSE96654 |
Epigenetic Drift of H3K27me3 in Aging Links Glycolysis to Healthy Longevity |
|
| Relations |
| BioSample |
SAMN06672320 |
| SRA |
SRX2694966 |
