|
| Status |
Public on Nov 30, 2017 |
| Title |
Pancreas_LmnaKO_6days_rep3 |
| Sample type |
RNA |
| |
|
| Source name |
Pancreas
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57 BL6
Sex: Male
genotype/variation: LmnaKO
|
| Treatment protocol |
Tamoxifen was administed at a dose of 3mg/40g by IP injection
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Combination of Trizol and Qiagen Rneasy protocols
|
| Label |
biotin
|
| Label protocol |
Biotinylated cDNA were prepared according to the Affymetrix Plus WT kit protocol (GeneChip® WT Plus Reagent Kit Manual P/N 703174 Rev. 2) from 100 ng total RNA
|
| |
|
| Hybridization protocol |
Following labeling procedure, 2.76 ug of cDNA were hybridized at 48C on Mouse Gene ST 2.1 arrays, and were washed and stained using the Affymetrix Gene Atlas system (software version 1.0.4.267)
|
| Scan protocol |
Arrays were scanned using the Affymetrix Gene Atlas system (software version 1.0.4.267)
|
| Data processing |
Data was analyzed using the limma and oligo Bioconductor packages implemented in the R-statistical environment v3.1.1. RMA was used to fit log2 transformed expression values
|
| |
|
| Submission date |
Apr 04, 2017 |
| Last update date |
Nov 30, 2017 |
| Contact name |
Juliana Bragazzi Cunha |
| E-mail(s) |
bragazzi@umich.edu
|
| Phone |
734-647-6461
|
| Organization name |
University of Michigan
|
| Department |
Molecular & Integrative Physiology
|
| Lab |
Omary Lab
|
| Street address |
1137 E Catherine St, MSII, room 7720
|
| City |
Ann Arbor |
| State/province |
MI |
| ZIP/Postal code |
48109 |
| Country |
USA |
| |
|
| Platform ID |
GPL17400 |
| Series (1) |
| GSE97396 |
Lamin A/C ablation in pancreatic acinar cells |
|
