|
| Status |
Public on Aug 22, 2017 |
| Title |
LAP+/OC.2+ E19 fetal hepatocytes bio rep 2 |
| Sample type |
RNA |
| |
|
| Source name |
LAP+/OC.2+ E19 fetal hepatocytes
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain: F344
age: Embryonic Day 19
tissue: Liver
cell type: LAP+/OC.2+ E19 fetal hepatocytes
cell isolation: Collagenase digestion, Immunopurification with monoclonal antibodies against leucine amino peptidase and OC.2. AutoMacs purified with double positive selection program
|
| Treatment protocol |
RNA was extracted from freshly isolated cells. The methodology for immunopurification of cells is described in Sanders JA. Late gestation fetal hepatocytes for repopulation in the rat. Methods Mol Biol. 2017;1506:45-60
|
| Growth protocol |
All animals were housed under standard conditions with access to food and water ad libitum. See sample columns for treatment protocols related to the different experimental groups.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using the Mirvana kit according to the manufacturer's directions.
|
| Label |
SAPE (streptavidin-conjugated Phycoerythrin)
|
| Label protocol |
Affymetrix WT amplification protocol was performed according to manufacturer's instructions. 150 ng total RNA input at start of RT rx, fragmentation: 5.5ug dUTP labeled sscDNA/rx, labeling: ~5.15 ug/rx
|
| |
|
| Hybridization protocol |
~ 2.3 ug/array, 17 h, 45 C, 60 rpm, Fluidics protocol FS450_0001
|
| Scan protocol |
Arrays were scanned using an Affymetrix GeneScanner 3000 7G
|
| Description |
LAP-OC2+ 2
LAP-OC.2+ 2
|
| Data processing |
Partek Genomics Suite, version 6.6, RMA ANOVA with contrast, 0.05 % FDR correction
|
| |
|
| Submission date |
Apr 17, 2017 |
| Last update date |
Aug 23, 2017 |
| Contact name |
Jennifer Ann Sanders |
| E-mail(s) |
Jennifer_Sanders@brown.edu
|
| Organization name |
Rhode Island Hospital
|
| Department |
Pediatric Endocrinology
|
| Street address |
593 Eddy Street
|
| City |
Providence |
| State/province |
RI |
| ZIP/Postal code |
02903 |
| Country |
USA |
| |
|
| Platform ID |
GPL6247 |
| Series (1) |
| GSE97897 |
Engraftment and Repopulation Potential of Late Gestation Fetal Rat Hepatocytes |
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