|
| Status |
Public on Sep 01, 2008 |
| Title |
benomyl_20min SC |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Total RNA from benomyl treated cells labeled with Cyanine-5 (red).
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
Strain BY4742, 20min after benomyl addition in growth media
|
| Treatment protocol |
Cells were grown to OD600nm = 0.6 and then treated with benomyl (Sigma-Aldrich) to a final concentration of 20μg/ml (stock solution: 10 mg/ml in DMSO). The mock treatment was done with an equivalent volume of DMSO. The cells were snapped- frozen in cold ethanol (final concentration 70% at -80°C) after 2, 4, 10, 20, 40 and 80 minutes of benomyl or mock treatment.
|
| Growth protocol |
All experiments were conducted in YPD rich media (2% glucose, 1% bactopeptone, 1% yeast extract).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Complete protocol can be found online : http://www.transcriptome.ens.fr/sgdb/protocols/preparation_yeast.php
|
| Label |
Cy5
|
| Label protocol |
Complete protocol can be found online : http://www.transcriptome.ens.fr/sgdb/protocols/labelling_yeast.php
|
| |
|
| Channel 2 |
| Source name |
Total RNA from mock treated cells labeled with Cyanine-3 (green).
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
Strain BY4742, 20min after DMSO addition in growth media
|
| Treatment protocol |
Cells were grown to OD600nm = 0.6 and then treated with benomyl (Sigma-Aldrich) to a final concentration of 20μg/ml (stock solution: 10 mg/ml in DMSO). The mock treatment was done with an equivalent volume of DMSO. The cells were snapped- frozen in cold ethanol (final concentration 70% at -80°C) after 2, 4, 10, 20, 40 and 80 minutes of benomyl or mock treatment.
|
| Growth protocol |
All experiments were conducted in YPD rich media (2% glucose, 1% bactopeptone, 1% yeast extract).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Complete protocol can be found online : http://www.transcriptome.ens.fr/sgdb/protocols/preparation_yeast.php
|
| Label |
Cy3
|
| Label protocol |
Complete protocol can be found online : http://www.transcriptome.ens.fr/sgdb/protocols/labelling_yeast.php
|
| |
|
| |
| Hybridization protocol |
Complete protocol can be found online : http://www.transcriptome.ens.fr/sgdb/protocols/hybridisation_yeast.php
|
| Scan protocol |
The microarrays were read using a Genepix 4000B scanner (Axon) and analyzed with the Genepix 6.0 software.
|
| Description |
Time = 20min
|
| Data processing |
Artefactual and saturated signal spots were eliminated. After image quantification, data were normalized over all features with print-tip lowess using the R/BioConductor packages “limma” and “marray” available at http://www.bioconductor.org/.
|
| |
|
| Submission date |
Jan 23, 2008 |
| Last update date |
Jan 23, 2008 |
| Contact name |
Gaëlle Lelandais |
| E-mail(s) |
lelandais@ebgm.jussieu.fr
|
| Organization name |
INSERM
|
| Street address |
2, place Jussieu
|
| City |
Paris |
| ZIP/Postal code |
75005 |
| Country |
France |
| |
|
| Platform ID |
GPL6384 |
| Series (1) |
|
