|
| Status |
Public on Apr 25, 2017 |
| Title |
Oocyte_rep4 |
| Sample type |
RNA |
| |
|
| Source name |
Human MII Oocyte
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Human MII Oocyte
maturation stage: Metaphase II
patients: Women undergone to ovarian stimulation for ICSI
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from fluids using the Qiagen miRNeasy Mini Kit (Qiagen GmbH). For RNA isolation from single oocyte see previous published protocols (PMID: 18765004)
|
| Label |
FAM
|
| Label protocol |
n/a
|
| |
|
| Hybridization protocol |
n/a
|
| Scan protocol |
n/a
|
| Description |
Control
|
| Data processing |
MiRNA expression profiles were analyzed using real-time RQ Manager software v1.2 (Applied Biosystems). Statistically significant miRNA differences were identified by Significance of Microarrays Analysis (SAM). MiRNA expression changes were calculated by applying the 2−ΔΔCT method and using the average Ct of each plate as endogenous controls.
DCt means of FF and oocytes were used to determine DDCt; oocytes were chosen as Calibrator. miRNA expression levels are reported as the natural logarithm of RQ values
|
| |
|
| Submission date |
Apr 24, 2017 |
| Last update date |
Apr 25, 2017 |
| Contact name |
Cinzia Di Pietro |
| E-mail(s) |
dipietro@unict.it
|
| Phone |
+390953782075
|
| Organization name |
University of Catania
|
| Department |
Department of Biomedical and Biotechnological Sciences
|
| Lab |
Biology and Genetics
|
| Street address |
via S.Sofia 87
|
| City |
Catania |
| ZIP/Postal code |
95123 |
| Country |
Italy |
| |
|
| Platform ID |
GPL17039 |
| Series (1) |
| GSE98103 |
MicroRNA profiling of human follicular fluid and MII oocytes |
|
