|
| Status |
Public on Jan 23, 2018 |
| Title |
GBS_A10033 |
| Sample type |
RNA |
| |
|
| Source name |
fetal cardiac tissue
|
| Organism |
Macaca nemestrina |
| Characteristics |
developmental stage: fetal
tissue: heart
treatment: Group B streptococcus
treatment location: Choriodecidual inoculation
labor: preterm
|
| Biomaterial provider |
University of Washington (Seattle, WA).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted from tissue samples using the Qiagen RNeasy kit, following the manufacturer's recommendations. Quality and quantity were assessed using an Agilent 2100 Bioanalyzer and NanoDrop 8000 spectrophotometer, respectively.
|
| Label |
Cy3
|
| Label protocol |
For microarray hybridization, RNA was processed for hybridization to Affymetrix Rhesus Macaque Array through a core facility in the Functional Genomics Laboratory in the Center for Ecogenetics and Environmental Health at the University of Washington according to the manufacturer's instructions.
|
| |
|
| Hybridization protocol |
The standard hybridization protocol was used as recommended by Affymetrix
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneArray Scanner 3000 through the University of Washington Functional Genomics Laboratory in the Center for Ecogenetics and Environmental Health.
|
| Data processing |
RMA normalization log2 transformed, using the oligo package of Bioconductor
|
| |
|
| Submission date |
May 02, 2017 |
| Last update date |
Jan 24, 2018 |
| Contact name |
James William MacDonald |
| E-mail(s) |
jmacdon@uw.edu
|
| Organization name |
University of Washington
|
| Department |
Environmental and Occupational Health Sciences
|
| Street address |
4225 Roosevelt Way NE
|
| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98105-6099 |
| Country |
USA |
| |
|
| Platform ID |
GPL21213 |
| Series (1) |
| GSE98459 |
Fetal sepsis in utero induces disruption of gene networks involved in cardiac morphogenesis in a nonhuman primate model of infection-induced preterm labor |
|
