|
| Status |
Public on Dec 03, 2017 |
| Title |
Healthy control 3 |
| Sample type |
RNA |
| |
|
| Source name |
oral cavity
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Oral mucosa
gender: male
age: 18
smoker: no
cigarettes (number/day): 0
localization: oral mucosa
|
| Extracted molecule |
total RNA |
| Extraction protocol |
We have extracted totalRNA from frozen tissue samples using the miRVana Isolation kit following the manufacturers instructions except the first part where we have disgregated the samples using diamond drill and hand piece. We have measured the concentration with the NanoDrop and the integrity of RNAtotal with the NanoChip and Bioanalyzer 2100, where all the samples had at least a RIN of 7
|
| Label |
biotin
|
| Label protocol |
We have used the Affymetrix® FlashTagTM Biotin HSR RNA Labeling Kit following the manufacturer instructions
|
| |
|
| Hybridization protocol |
We have used Affymetrix®miRNA 4.1 Array Plate
|
| Scan protocol |
we have used GeneTitan®Instrument
|
| Data processing |
we have used Affymetrix®GeneChip® Command Console® (AGCC) and finally the trancriptome analysis console (TAC)
|
| |
|
| Submission date |
May 02, 2017 |
| Last update date |
Jan 23, 2018 |
| Contact name |
Cintia Micaela Chamorro |
| E-mail(s) |
cintiamica.chamo@yahoo.es
|
| Phone |
0034651011815
|
| Organization name |
Santiago de Compostela University
|
| Department |
Oral Medicin
|
| Street address |
Entrerríos s/n
|
| City |
Santiago de Compostela |
| ZIP/Postal code |
15705 |
| Country |
Spain |
| |
|
| Platform ID |
GPL21572 |
| Series (1) |
| GSE98463 |
Differential Expression of microRNAs in Oral Squamous cell carcinoma (OSCC) [Frozen tissue samples] |
|
