|
| Status |
Public on Mar 22, 2009 |
| Title |
SQ20B Go6976 12hr 2 |
| Sample type |
other |
| |
|
| Channel 1 |
| Source name |
12hr 2
|
| Organism |
Homo sapiens |
| Characteristics |
SQ20B cells 12hr 100nm Gö6976
|
| Treatment protocol |
control, 12 or 24hr 100nm Gö6976 exposure
|
| Growth protocol |
SQ20B (5 x 10e6) tumor cells were injected into the right hind limbs of female athymic nude mice
|
| Extracted molecule |
other |
| Extraction protocol |
PureLink miRNA Isolation Kit (Invitrogen, Carlsbad, CA)
|
| Label |
Cy3
|
| Label protocol |
mirVana miRNA labeling kit (Ambion, Austin, TX)
|
| |
|
| Channel 2 |
| Source name |
0hr
|
| Organism |
Homo sapiens |
| Characteristics |
SQ20B cells control
|
| Treatment protocol |
control, 12 or 24hr 100nm Gö6976 exposure
|
| Growth protocol |
SQ20B (5 x 10e6) tumor cells were injected into the right hind limbs of female athymic nude mice
|
| Extracted molecule |
other |
| Extraction protocol |
PureLink miRNA Isolation Kit (Invitrogen, Carlsbad, CA)
|
| Label |
Cy5
|
| Label protocol |
mirVana miRNA labeling kit (Ambion, Austin, TX)
|
| |
|
| |
| Hybridization protocol |
manufacturer's specification
|
| Scan protocol |
scanned using Axon 4000B and image analysis using GenePix Pro 4.0
|
| Description |
6 treated samples across two time points hybridized to paired time 0, untreated controls
|
| Data processing |
loess normalization (R - Bioconductor 'limma' package)
|
| |
|
| Submission date |
Jan 28, 2008 |
| Last update date |
Mar 22, 2009 |
| Contact name |
Christine H. Chung |
| E-mail(s) |
christine.chung@vanderbilt.edu
|
| Phone |
615-322-4967
|
| Fax |
615343-7602
|
| Organization name |
Vanderbilt University
|
| Department |
Internal Medicine
|
| Lab |
Chung
|
| Street address |
777 PRB
|
| City |
Nashville |
| State/province |
TN |
| ZIP/Postal code |
37232-6307 |
| Country |
USA |
| |
|
| Platform ID |
GPL6429 |
| Series (1) |
| GSE10301 |
Protein Kinase C Alpha Promotes Tumor Growth by Initiating a Feed-forward Network Involving miR-15a and Cyclin E |
|
