|
| Status |
Public on Jun 01, 2017 |
| Title |
Experimental 4 |
| Sample type |
SRA |
| |
|
| Source name |
Mouse Hypothalamus
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57B/L6
genotype: Thrb-flox
treatment: AAV-CRE
|
| Treatment protocol |
Thrb-flox mice were injected with either GFP/Cre overexpressing adeno-associated viruses centrally to remove ThrB expression in the hypothalamus and animals were sacrificed 4 weeks after injection
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted from microdissected mouse hypothalami 4 weeks after AAV injection
Illumina TruSeq mRNA sample prep kit was used to make sequencing libraries
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Description |
Mouse Hypothalamus injected with AAV-CRE - Experimental 4
IGF0004619
|
| Data processing |
Reads were aligned to GRCm38 using Tophat (v 0.11.2)
Counts were generated using HTSeq (v 0.6.0)
Differential expression analysis was performed using EdgeR.
EdgeR was used to generate (TMM) normalised abundance values in log2CPM
Genome_build: GRcm38
Supplementary_files_format_and_content: Normalised abundance values are represented in log2 counts per million (log2CPM) and stored in a tab-delimited matrix
|
| |
|
| Submission date |
May 09, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Brian Yee Hong Lam |
| E-mail(s) |
yhbl2@cam.ac.uk
|
| Organization name |
University of Cambridge
|
| Department |
Metabolic Research Laboratories
|
| Street address |
Box 289 Addenbookes Hospital, Hills Road
|
| City |
Cambridge |
| ZIP/Postal code |
CB2 0QQ |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL17021 |
| Series (1) |
| GSE98690 |
Thyroid hormone receptor beta in the ventromedial hypothalamus is essential for the physiological regulation of food intake and body weight |
|
| Relations |
| BioSample |
SAMN06920642 |
| SRA |
SRX2792547 |
