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| Status |
Public on May 11, 2017 |
| Title |
NK cells, 5 ng/mlTGF-b1_donor2 |
| Sample type |
RNA |
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| Source name |
Blood, NK cells
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| Organism |
Homo sapiens |
| Characteristics |
tissue: blood
cell type: NK cells
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| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA containing the small RNA fraction was extracted using the miRCURY RNA Isolation Kit - Cell and Plant (Exiqon)
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| Label |
FAM
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| Label protocol |
Total RNA was reverse transcribed using the TaqMan MicroRNA Reverse Transcription kit with the Megaplex RT Primers Human Pool A+B set v.3.0 (catalog number 4444913, Applied Biosystems), a preamplification step was performed using the TaqMan PreAmp Master Mix with the Megaplex PreAmp Primers Human Pool A+B set v.3.0, (Applied Biosystems) preamplified cDNAs were loaded in TaqMan Array Human MicroRNA Cards A and B set v3.0 (Applied Biosystems).
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| Hybridization protocol |
n/a
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| Scan protocol |
n/a
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| Description |
Test
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| Data processing |
Data were analyzed using the miScript miRNA PCR Array Data Analysis Tool (http://www.sabiosciences.com/mirnaArrayDataAnalysis.php). MiRNAs expression was normalized to the U6 snRNA expression, for which four replicate wells are present in each array card. The ΔΔCt method was used. The web-based software automatically performed all calculations from the uploaded raw threshold cycle data.
normalized data file reports normalized signal (against reference U6 snRNA). Fold Change file reports test/control (i.e., treated NK/untreated NK) ratios.
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| Submission date |
May 10, 2017 |
| Last update date |
May 11, 2017 |
| Contact name |
Stefano Regis |
| E-mail(s) |
stefanoregis@gaslini.org
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| Organization name |
Istituto G. Gaslini
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| Street address |
Via G. Gaslini 5
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| City |
Genova |
| ZIP/Postal code |
16147 |
| Country |
Italy |
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| Platform ID |
GPL18942 |
| Series (1) |
| GSE98769 |
MiRNA expression variation in primary NK cells treated with TGF-β1 |
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| Supplementary data files not provided |
| Processed data are available on Series record |
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