|
| Status |
Public on Feb 02, 2008 |
| Title |
Idenitfication of differentially expressed genes in Runx1-/- primitive erythroid |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
WT
|
| Organism |
Mus musculus |
| Characteristics |
strain:C57/BL6
age:E10.5
tissue:yolk sac
genotype:runx1+/+
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA from E10.5 wild type yolk sacs was extracted using RNeasy kit (Qiagen), and three samples were mixed equally. The quality of the isolated RNA was determined using an Agilent 2100 Bioanalyzer (Agilent Technologies).
|
| Label |
Cy3
|
| Label protocol |
Total RNA was labeled using a Low Input Fluorescent Linear Amplification kit (Agilent) following the manufacturer’s instruction.
|
| |
|
| Channel 2 |
| Source name |
runx1-/-
|
| Organism |
Mus musculus |
| Characteristics |
strain:C57/BL6
age:E10.5
tissue:yolk sac
genotype:runx1-/-
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA from E10.5 runx1-/- yolk sacs was extracted using RNeasy kit (Qiagen), and three samples were mixed equally. The quality of the isolated RNA was determined using an Agilent 2100 Bioanalyzer (Agilent Technologies).
|
| Label |
cy5
|
| Label protocol |
Total RNA was labeled using a Low Input Fluorescent Linear Amplification kit (Agilent) following the manufacturer’s instruction.
|
| |
|
| |
| Hybridization protocol |
17 hour incubation following Agilent protocols using 750ng of both Cy3 and Cy5 labeled material, In Situ Hybridization Kit reagents and control probes followed by a 10 minute room temperature wash in 6X SSC,0.005% tritonX-102 and 5 minutes at 4 degrees C in 0.1X SSC, 0.005% Triton X-102. The arrays were dried in a Nitrogen stream and scanned.
|
| Scan protocol |
The scanning was performed with an Agilent Technologies Microarray Scanner (Agilent Technologies).
|
| Description |
none
|
| Data processing |
The log ratio of the red over green processed signals is calculated.
|
| |
|
| Submission date |
Feb 01, 2008 |
| Last update date |
Feb 29, 2024 |
| Contact name |
Kazuteru Hasegawa |
| E-mail(s) |
kazuteru@stanford.edu
|
| Organization name |
Stanford University
|
| Street address |
Lorry Lokey Stem Cell Research Building (SIM1), G2165 265 Campus Drive
|
| City |
Stanford |
| ZIP/Postal code |
94305 |
| Country |
USA |
| |
|
| Platform ID |
GPL922 |
| Series (1) |
| GSE10348 |
Identification of differentially expressed genes in runx1-/- primitive erythroid |
|
