|
| Status |
Public on Oct 01, 2019 |
| Title |
TMA-ARB-9 |
| Sample type |
SRA |
| |
|
| Source name |
MCa-M3C breast tumors
|
| Organism |
Mus musculus |
| Characteristics |
tissue: MCa-M3C breast tumors
cell line: MCa-M3C
host mouse strain: FVB/N
|
| Treatment protocol |
40 mg/kg valsartan in the TMA-ARB form or an equal volume of PBS intravenously (retro-orbital injection) each day for 6 days
|
| Growth protocol |
Mice bearing orthotopic MCa-M3C tumors were split into time- and size-matched (~100 mm^3) treatment groups
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
Total RNA extraction and poly-A selection with NEBNext Poly(A) mRNA Magnetic Isolation Module (E7490L, NEB).
NEBNext Ultra Directional RNA Library Prep Kit (E7420L, NEB)
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Description |
Sample name: MAg20-9
|
| Data processing |
Alignment and transcript quantification: Using the aligner Salmon (http://salmon.readthedocs.io/en/latest/salmon.html) under default filtering settings, sequencing reads (fastqs) were aligned to, and count estimates calculated for, GenCode- annotated mouse (mm10, vM9) transcripts.
Genome_build: mm10
Supplementary_files_format_and_content: Raw counts generated by Salmon in txt format
|
| |
|
| Submission date |
May 11, 2017 |
| Last update date |
Oct 01, 2019 |
| Contact name |
Kamila Naxerova |
| Organization name |
Massachusetts General Hospital / Harvard Medical School
|
| Department |
Center For Systems Biology
|
| Street address |
149 13th Street
|
| City |
Charlestown |
| State/province |
MA |
| ZIP/Postal code |
02129 |
| Country |
USA |
| |
|
| Platform ID |
GPL13112 |
| Series (1) |
| GSE98827 |
Tumor microenvironment-activated angiotensin blockers enhance cancer immunotherapy |
|
| Relations |
| BioSample |
SAMN06929332 |
| SRA |
SRX2797835 |
