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Status |
Public on Oct 06, 2020 |
Title |
Tax1BP1 knock-out 3 [miRNA] |
Sample type |
RNA |
|
|
Source name |
Tax1BP1 knock-out
|
Organism |
Mus musculus |
Characteristics |
strain background: C57BL/6 genotype/variation: Tax1BP1 knock-out age: 12 weeks Sex: male tissue: liver
|
Treatment protocol |
no treatment
|
Growth protocol |
Mice were housed under 12 h day/night cycles, were fed standard rodent chow and had access to drinking water ad libitum.
|
Extracted molecule |
total RNA |
Extraction protocol |
miRNA was extracted with the miRNeasy Mini Kit from Qiagen
|
Label |
biotin
|
Label protocol |
miRNA was labeled with the Affymetrix FlashTag Biotin HSR RNA Labeling Kit
|
|
|
Hybridization protocol |
GeneChip® miRNA 3.0 arrays (Affymetrix) were hybridized with 500 ng labelled microRNA, Hybridisation time was 18 hours.
|
Scan protocol |
Affymetrix Gene ChIP Scanner 3000 7G
|
Description |
1607_(miRNA-3_0).CEL
|
Data processing |
Statistical analysis was done with the statistical computing environment R. Additional software packages (affy, geneplotter, multtest, and vsn) were taken from the Bioconductor project. Microarray preprocessing: Probe level normalization was conducted using the variance stabilization method (vsn) from Huber et al.. probe group file: UnsupportedGeneChip® miRNA 3.0 Array CDF Files meta-probeset file: miRNA 3.0 Annotations, Unsupported, CSV format from Affymetrix
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|
|
Submission date |
May 15, 2017 |
Last update date |
Oct 06, 2020 |
Contact name |
Claudia Döring |
E-mail(s) |
C.Doering@em.uni-frankfurt.de
|
Organization name |
Johann Wolfgang-Goethe University Frankfurt a.M.
|
Department |
Medical School, Institite of Pathology
|
Street address |
Theodor Stern Kai 7
|
City |
Frankfurt |
ZIP/Postal code |
60590 |
Country |
Germany |
|
|
Platform ID |
GPL16384 |
Series (2) |
GSE98907 |
Differential expression of miRNA in Tax1BP1 knock-out mice [miRNA] |
GSE98909 |
Differential expression of miRNA and mRNA in Tax1BP1 knock-out mice |
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