|
| Status |
Public on May 27, 2017 |
| Title |
NS_277 |
| Sample type |
RNA |
| |
|
| Source name |
gliomasphere culture
|
| Organism |
Homo sapiens |
| Characteristics |
diagnosis: GBM Secondary (may be recurrent)
days: 1517
d945: --
|
| Treatment protocol |
RNA was isolated from gliomaspheres and tumors samples using the Qiagen RNA isolation kit (RNeasy Kit, Qiagen).
|
| Growth protocol |
Cells were plated at 5000 cells/100mL/well of a 96-well plate and grown for 5-7 days. Cell number was assessed using Dojindo Cell Counting Kit-8 (Dojindo Molecular Technolo-gies Inc.)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol,concentration and quality of RNA samples was examined using the NanoDrop ND-1000 spectrophotometer (NanoDrop Tech- nologies) and the Agilent 2100 Bioanalyzer (Agilent Technologies). RNA samples were reverse transcribed and labeled according to manufacturer’s instructions
|
| |
|
| Hybridization protocol |
Not provided
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Data processing |
Data were background corrected, log2 transformed, and quantile normalized with Robust Multi-array Average (RMA) method.
|
| |
|
| Submission date |
May 17, 2017 |
| Last update date |
Jan 23, 2018 |
| Contact name |
Giovanni Coppola |
| E-mail(s) |
gcoppola@ucla.edu
|
| Phone |
310-794-4172
|
| Organization name |
UCLA
|
| Department |
Psychiatry and Neurology
|
| Lab |
Neurogenetics
|
| Street address |
1524 Gonda, 695 Charles Young Drive South
|
| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90095 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE98995 |
Large-scale assessment of the gliomasphere model system |
|
