|
| Status |
Public on Jul 15, 2017 |
| Title |
Nt_artificial_precursor_rep2 |
| Sample type |
SRA |
| |
|
| Source name |
infiltrated leaves
|
| Organism |
Nicotiana tabacum |
| Characteristics |
genotype: Wisconsin 35
material: Leaves transiently expressing GFP under 35s promoter
tissue: leaves
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA from infiltrated leaves was extracted using Trizol (Invitrogen) reagent.
Size fractionation
|
| |
|
| Library strategy |
ncRNA-Seq |
| Library source |
transcriptomic |
| Library selection |
size fractionation |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Data processing |
3’ adapter (5’-TGGAATTCTCGGGTGCCAAGG-3’) was removed from sequenced reads using UEA small RNA workbench.
The small RNA reads of length 18nt-25nt filtered and were analyzed.
Supplementary_files_format_and_content: Text file containing 18nt-25nt long unique reads and their raw abundance after removal of adapter.
|
| |
|
| Submission date |
May 19, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Shivaprasad Padubidri V. |
| E-mail(s) |
shivaprasad@ncbs.res.in
|
| Phone |
+918023666511
|
| Organization name |
National Centre for Biological Sciences, TIFR
|
| Street address |
GKVK Campus, Bellary Road
|
| City |
Bengaluru |
| State/province |
Karnataka |
| ZIP/Postal code |
560065 |
| Country |
India |
| |
|
| Platform ID |
GPL23495 |
| Series (1) |
| GSE99094 |
Understanding the structure/sequence preference of plant miRNA biogenesis machinery |
|
| Relations |
| BioSample |
SAMN07144853 |
| SRA |
SRX2836614 |
