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Sample GSM2641254 Query DataSets for GSM2641254
Status Public on Aug 12, 2017
Title Membranous Glomerulonephritis [H5-Tub-MGN356]
Sample type RNA
 
Source name Kidney Biopsy from Membranous Glomerulonephritis
Organism Homo sapiens
Characteristics tissue: tubulo interstitium from kidney biopsy
batch: 5
Treatment protocol none
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using Rneasy kit (Qiagen) following manufacturer's protocol
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from total RNA (Expression Analysis Technical Manual, Affymetrix).
 
Hybridization protocol GeneChip arrays were scanned by GeneChip Scanner 3000 7G according to the Affymetrix Expression Analysis Technical Manual.
Scan protocol The data were analyzed using GenePattern pipeline: RMA method and Human Entrez Gene custom CDF annotation version 18 was used for the normalization. Background data was filtered by excluding all genes that did not have at least one expression value exceeding the median plus 2 standard deviations of the Affymetrix negative control expression.
Description Gene Expression data from the tubulointerstitial compartment of human Membranous Glomerulonephritis [H5-Tub-MGN356]
Data processing Each of the hybridization was normalized seperately and the last sheet contains the batchcorrected data using Combat
 
Submission date May 25, 2017
Last update date Jan 23, 2018
Contact name Felix Eichinger
E-mail(s) eichinge@umich.edu
Organization name The University of Michigan
Department Division of Nephrology
Street address 1150 W. Medical Center Drive
City Ann Arbor
State/province Michigan
ZIP/Postal code 48109
Country USA
 
Platform ID GPL19184
Series (2)
GSE99325 Transcriptome-based network analysis reveals renal cell type-specific dysregulation of hypoxia-associated transcripts [Tub-FE]
GSE99340 Transcriptome-based network analysis reveals renal cell type-specific dysregulation of hypoxia-associated transcripts

Data table header descriptions
ID_REF
VALUE Entrez Gene IDs and Log base 2 expression values

Data table
ID_REF VALUE
2_at 9.736046471
9_at 8.01600937
10_at 6.026462296
12_at 7.536092274
13_at 5.024978946
14_at 8.359605036
15_at 5.171301519
16_at 9.269348099
18_at 9.487964954
19_at 9.42372509
20_at 5.266345453
21_at 5.812005313
22_at 7.638117954
23_at 8.810725903
24_at 5.739200905
25_at 8.217478297
26_at 10.48631879
27_at 5.689446894
28_at 5.459451214
29_at 5.817003846

Total number of rows: 10644

Table truncated, full table size 211 Kbytes.




Supplementary file Size Download File type/resource
GSM2641254_H5-Tub-MGN356.CEL.gz 2.2 Mb (ftp)(http) CEL
Processed data included within Sample table

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