|
| Status |
Public on Aug 12, 2017 |
| Title |
Systemic Lupus Erythematosus [H5-Tub-SLE338] |
| Sample type |
RNA |
| |
|
| Source name |
Kidney Biopsy from Systemic Lupus Erythematosus
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: tubulo interstitium from kidney biopsy
batch: 5
|
| Treatment protocol |
none
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using Rneasy kit (Qiagen) following manufacturer's protocol
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from total RNA (Expression Analysis Technical Manual, Affymetrix).
|
| |
|
| Hybridization protocol |
GeneChip arrays were scanned by GeneChip Scanner 3000 7G according to the Affymetrix Expression Analysis Technical Manual.
|
| Scan protocol |
The data were analyzed using GenePattern pipeline: RMA method and Human Entrez Gene custom CDF annotation version 18 was used for the normalization. Background data was filtered by excluding all genes that did not have at least one expression value exceeding the median plus 2 standard deviations of the Affymetrix negative control expression.
|
| Description |
Gene Expression data from the tubulointerstitial compartment of human Systemic Lupus Erythematosus [H5-Tub-SLE338]
|
| Data processing |
Each of the hybridization was normalized seperately and the last sheet contains the batchcorrected data using Combat
|
| |
|
| Submission date |
May 25, 2017 |
| Last update date |
Jan 23, 2018 |
| Contact name |
Felix Eichinger |
| E-mail(s) |
eichinge@umich.edu
|
| Organization name |
The University of Michigan
|
| Department |
Division of Nephrology
|
| Street address |
1150 W. Medical Center Drive
|
| City |
Ann Arbor |
| State/province |
Michigan |
| ZIP/Postal code |
48109 |
| Country |
USA |
| |
|
| Platform ID |
GPL19184 |
| Series (2) |
| GSE99325 |
Transcriptome-based network analysis reveals renal cell type-specific dysregulation of hypoxia-associated transcripts [Tub-FE] |
| GSE99340 |
Transcriptome-based network analysis reveals renal cell type-specific dysregulation of hypoxia-associated transcripts |
|
