|
| Status |
Public on Aug 11, 2017 |
| Title |
T32 |
| Sample type |
RNA |
| |
|
| Source name |
DMPM frozen tumor specimen
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: DMPM frozen tumor specimen
telomerase activity: 0
|
| Treatment protocol |
na
|
| Growth protocol |
na
|
| Extracted molecule |
total RNA |
| Extraction protocol |
For total RNA extraction, approximately 100 mg of frozen tissue was homogenized using a bench-top homogenizer (MM200, Retsch, Germany) in 1 mL of TRIzol Reagent (Invitrogen).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cDNA was prepared with the TotalPrep Amplification kit (Ambion) for Illumina arrays
|
| |
|
| Hybridization protocol |
Fluorescently labelled cDNA PCR products were hybridized to the Illumina Sentrix BeadChip according to the protocols provided by the manufacturer.
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
4616793027_E
|
| Data processing |
Array data export processing and analysis was performed using Illumina BeadStudio v3.1.3 (Gene Expression Module v3.3.8). Raw expression data were log2-transformed and normalized using the robust spline normalization method.
|
| |
|
| Submission date |
May 26, 2017 |
| Last update date |
Aug 11, 2017 |
| Contact name |
Paolo Gandellini |
| E-mail(s) |
paolo.gandellini@istitutotumori.mi.it
|
| Organization name |
Fondazione IRCCS Istituto Nazionale Tumori
|
| Department |
Department of Applied Research and Technological Development
|
| Street address |
Via Amadeo 42
|
| City |
Milan |
| ZIP/Postal code |
20133 |
| Country |
Italy |
| |
|
| Platform ID |
GPL8179 |
| Series (1) |
| GSE99362 |
microRNA expression profiling in diffuse malignant peritoneal mesothelioma |
|
