|
Status |
Public on Sep 27, 2018 |
Title |
AMO-1 |
Sample type |
RNA |
|
|
Source name |
cell line without treatment
|
Organism |
Homo sapiens |
Characteristics |
disease: Multiple myeloma (MM)
|
Treatment protocol |
not applicaple
|
Growth protocol |
In tissue culture media supplemented with pen/strep and fetal calf serum (10-20 %). Incubated with 5% CO2 at 37 degrees celcius
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was purified using Trizol and transferring to mirVANA columns (#1561, Ambion) and follow the manufacturer’s instruction
|
Label |
Biotin
|
Label protocol |
The following two kits from NuGEN were used following manufactures guide lines: The WT-Ovation™ Exon Module Version 1.0 and The Encore™Biotin Module
|
|
|
Hybridization protocol |
As described in the user guide, appendix A, of the The Encore™Biotin Module (NuGEN)
|
Scan protocol |
Affymetrix Gene ChIP Scanner 3000 7G. CEL-files generated using AGCC
|
Description |
gene expression data from untreated cell line
|
Data processing |
RMA normalized using R (v3.3.2), the affy-package (v1.48.0) from Bioconductor, and probe set data extracted from custom CDF [Brainarray, huex10sthsensgcdf_20.0.0]. Probe set data extracted from custom CDF [Brainarray, huex10sthsensgcdf_20.0.0] Probe set data extracted from custom CDF [Brainarray, huex10sthsensgcdf_20.0.0]
|
|
|
Submission date |
Jun 02, 2017 |
Last update date |
Sep 27, 2018 |
Contact name |
Rasmus Froberg Brøndum |
E-mail(s) |
rfb@rn.dk
|
Organization name |
Aalborg University Hospital
|
Department |
Department of Haematology
|
Street address |
Sdr. Skovvej 15
|
City |
Aalborg |
ZIP/Postal code |
9000 |
Country |
Denmark |
|
|
Platform ID |
GPL23541 |
Series (2) |
GSE99634 |
Gene expression profiles of multiple myeloma plasma cell fractions from bone marrow I |
GSE99636 |
Gene expression profiles of multiple myeloma plasma cell fractions from bone marrow |
|