|
| Status |
Public on Sep 27, 2018 |
| Title |
U-266 |
| Sample type |
RNA |
| |
|
| Source name |
cell line without treatment
|
| Organism |
Homo sapiens |
| Characteristics |
disease: Multiple myeloma (MM)
|
| Treatment protocol |
not applicaple
|
| Growth protocol |
In tissue culture media supplemented with pen/strep and fetal calf serum (10-20 %). Incubated with 5% CO2 at 37 degrees celcius
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was purified using Trizol and transferring to mirVANA columns (#1561, Ambion) and follow the manufacturer’s instruction
|
| Label |
Biotin
|
| Label protocol |
The following two kits from NuGEN were used following manufactures guide lines: The WT-Ovation™ Exon Module Version 1.0 and The Encore™Biotin Module
|
| |
|
| Hybridization protocol |
As described in the user guide, appendix A, of the The Encore™Biotin Module (NuGEN)
|
| Scan protocol |
Affymetrix Gene ChIP Scanner 3000 7G. CEL-files generated using AGCC
|
| Description |
gene expression data from untreated cell line
|
| Data processing |
RMA normalized using R (v3.3.2), the affy-package (v1.48.0) from Bioconductor, and probe set data extracted from custom CDF [Brainarray, huex10sthsensgcdf_20.0.0].
Probe set data extracted from custom CDF [Brainarray, huex10sthsensgcdf_20.0.0]
Probe set data extracted from custom CDF [Brainarray, huex10sthsensgcdf_20.0.0]
|
| |
|
| Submission date |
Jun 02, 2017 |
| Last update date |
Sep 27, 2018 |
| Contact name |
Rasmus Froberg Brøndum |
| E-mail(s) |
rfb@rn.dk
|
| Organization name |
Aalborg University Hospital
|
| Department |
Department of Haematology
|
| Street address |
Sdr. Skovvej 15
|
| City |
Aalborg |
| ZIP/Postal code |
9000 |
| Country |
Denmark |
| |
|
| Platform ID |
GPL23541 |
| Series (2) |
| GSE99634 |
Gene expression profiles of multiple myeloma plasma cell fractions from bone marrow I |
| GSE99636 |
Gene expression profiles of multiple myeloma plasma cell fractions from bone marrow |
|
