|
| Status |
Public on Jun 08, 2017 |
| Title |
LW3 |
| Sample type |
SRA |
| |
|
| Source name |
Embryonic longissimus dorsi muscle of pig
|
| Organism |
Sus scrofa |
| Characteristics |
breed: Large White pig
breed type: introduced pig breed
developmental stage: embryonic
tissue: Longissimus dorsi muscle
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Longissimus dorsi muscle were collected, flash frozen on dry ice, and RNA was harvested using Trizol reagent. TruSeq RNA Sample Preparation Guide (Part#15008136 Rev.A, Illumina) was used with 3 ug of total RNA for the construction of sequencing libraries.
RNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 4000 |
| |
|
| Data processing |
Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence using CLC Genomics Workbench(version:4.8)
Clean reads were aligned onto the whole reference genome (Sus scrofa 10.2) using TopHat
The number of fragments per kilobase of exon length million mapped reads (FPKM) analyzed by Cufflink (version 2.1.2) was used as the value of the normalized gene expression.
Genome_build: Sus scrofa 10.2
Supplementary_files_format_and_content: tab-delimited text files include FPKM values for TP WJ LW
|
| |
|
| Submission date |
Jun 07, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Liyuan Wang |
| E-mail(s) |
651312661@qq.com
|
| Phone |
+8618518153609
|
| Organization name |
China Agricultura University
|
| Department |
Animal Sciencie and Technology
|
| Lab |
Lab of Animal Genetic Resourse and Molecular Breeding
|
| Street address |
No.2 of Yuanmingyuan West Road, Haidian District
|
| City |
Beijing |
| State/province |
China |
| ZIP/Postal code |
+86-100094 |
| Country |
China |
| |
|
| Platform ID |
GPL22475 |
| Series (1) |
| GSE99749 |
Integrating transcriptome and proteome profiles of prenatal muscle tissue to reveal the postnatal growth differences in pigsof Pig Muscle Tissue |
|
| Relations |
| BioSample |
SAMN07201005 |
| SRA |
SRX2888272 |
