|
| Status |
Public on Jan 01, 2018 |
| Title |
quiescent NSC 48h after irradiation, replicate 3 |
| Sample type |
RNA |
| |
|
| Source name |
Adult mouse brain
|
| Organism |
Mus musculus |
| Characteristics |
tissue: subventricular zone
gender: male
strain: C57B6J
state: quiescent neural stem cells during the recovery phase 48h after radiation exposure
|
| Growth protocol |
Neural stem cells were freshly sorted from adult mouse brain
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA were extracted using Qiagen then transcribed into cDNA and 5 µg of cDNA were amplified using NuGEN Ovation Pico WTA System
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 µg total RNA.
|
| |
|
| Hybridization protocol |
Following fragmentation, 5 µg of cDNA were hybridized for 16 hr at 45°C on GeneChip MOE 430 2.0. GeneChips were washed and stained.
|
| Scan protocol |
GeneChips were scanned using GeneChip Scanner 3000.
|
| Description |
Gene expression data from quiescent neural stem cells during the recovery phase 48h after radiation exposure
|
| Data processing |
The .CEL files were generated using Affymetrix software and the data were analyzed using GeneSpring GX12. Sample 3 and Sample 12 did not pass QC.
|
| |
|
| Submission date |
Jun 07, 2017 |
| Last update date |
Jan 23, 2018 |
| Contact name |
Marc-André MOUTHON |
| E-mail(s) |
marc-andre.mouthon@cea.fr
|
| Organization name |
CEA
|
| Department |
IRCM
|
| Lab |
Laboratoire de Radiopathologie
|
| Street address |
Route du Panorama
|
| City |
Fontenay-aux-Roses |
| ZIP/Postal code |
92265 |
| Country |
France |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE99777 |
Expression data of adult quiescent and activated mouse neural stem cells |
|
