|
| Status |
Public on Aug 28, 2017 |
| Title |
D180CtrlEpdSC_R2_ATAC-seq |
| Sample type |
SRA |
| |
|
| Source name |
Skin
|
| Organism |
Mus musculus |
| Characteristics |
Sex: female
strain: C57BL/6
cell types: adult skin epithelia
sort markers: Integrin A6+, B1+ SCa1+ CD34-
|
| Treatment protocol |
starting at postnatal day 60 mice were treated with topical imiquimod (IMQ) on control vanicream and harvested at inticated timepoints; samples 9-16 30days post IMQ/vanicream mice were subject to full thickness wounds in dorsal skin
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Cells were FACS sorted according to characteristics: sort markers, and Nuclei were extracted and genomic DNA was transposed per protocl outlined in doi:10.1038/nmeth.2695
Nextera DNA Library Preparation Kit
|
| |
|
| Library strategy |
ATAC-seq |
| Library source |
genomic |
| Library selection |
other |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Description |
PCR amplified library from genomic DNA
|
| Data processing |
Reads were aligned to the reference mouse genome (mm10) with bowtie as previously described (Buenrostro 2013).
Genome_build: mm10
|
| |
|
| Submission date |
Jun 07, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Nicholas Gomez |
| Organization name |
Rockefeller University
|
| Street address |
1230 York Ave
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10065 |
| Country |
USA |
| |
|
| Platform ID |
GPL17021 |
| Series (1) |
| GSE92967 |
Inflammatory Memory in Epidermal Stem Cells Accelerates Tissue Damage Responses |
|
| Relations |
| BioSample |
SAMN07203514 |
| SRA |
SRX2893907 |
