|
Status |
Public on Jun 07, 2020 |
Title |
Vaginal pinch biopsy-Baseline-0.0-Fe-rep2 |
Sample type |
RNA |
|
|
Source name |
Vaginal pinch biopsy-Baseline-0.0-Fe
|
Organism |
Macaca nemestrina |
Characteristics |
tissue: Vaginal pinch biopsy animal: BB173 treatment: Baseline dose: 0 condition: early follicular
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA extraction was performed using the RNeasy mini kit (QIAGEN, Hilden, Germany)
|
Label |
biotin
|
Label protocol |
Five µg of the amplified cDNA was fragmented, biotinylated, and hybridized to arrays using the NuGEN Ovation Encore Biotin Module, according to kit instructions.
|
|
|
Hybridization protocol |
Arrays were washed, stained and scanned as described in the Affymetrix GeneChip® Expression Analysis Technical Manual
|
Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
|
Data processing |
Data from .CEL files were analyzed using the 'affy' Bioconductor package and Partek Genomics Suite.
|
|
|
Submission date |
Jun 08, 2017 |
Last update date |
Jun 07, 2020 |
Contact name |
Gregory K Tharp |
E-mail(s) |
gktharp@emory.edu
|
Phone |
404-727-7797
|
Organization name |
Yerkes National Primate Research Center
|
Department |
Developmental and Cognitive Neuroscience
|
Lab |
Genomics Core
|
Street address |
954 Gatewood Dr
|
City |
Atlanta |
State/province |
GA |
ZIP/Postal code |
30329-4208 |
Country |
USA |
|
|
Platform ID |
GPL3535 |
Series (1) |
GSE99800 |
Effects of Injectable DMPA and Combined Oral Contraceptive Use on HIV Susceptibility Factors in Pig-tailed Macaques |
|