|
| Status |
Public on Jun 07, 2020 |
| Title |
Vaginal pinch biopsy-DMPA-2.5-1D4-rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Vaginal pinch biopsy-DMPA-2.5-1D4
|
| Organism |
Macaca nemestrina |
| Characteristics |
tissue: Vaginal pinch biopsy
animal: BB0499
treatment: DMPA
dose: 2.5
condition: 1D4
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extraction was performed using the RNeasy mini kit (QIAGEN, Hilden, Germany)
|
| Label |
biotin
|
| Label protocol |
Five µg of the amplified cDNA was fragmented, biotinylated, and hybridized to arrays using the NuGEN Ovation Encore Biotin Module, according to kit instructions.
|
| |
|
| Hybridization protocol |
Arrays were washed, stained and scanned as described in the Affymetrix GeneChip® Expression Analysis Technical Manual
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
|
| Data processing |
Data from .CEL files were analyzed using the 'affy' Bioconductor package and Partek Genomics Suite.
|
| |
|
| Submission date |
Jun 08, 2017 |
| Last update date |
Jun 07, 2020 |
| Contact name |
Gregory K Tharp |
| E-mail(s) |
gktharp@emory.edu
|
| Phone |
404-727-7797
|
| Organization name |
Yerkes National Primate Research Center
|
| Department |
Developmental and Cognitive Neuroscience
|
| Lab |
Genomics Core
|
| Street address |
954 Gatewood Dr
|
| City |
Atlanta |
| State/province |
GA |
| ZIP/Postal code |
30329-4208 |
| Country |
USA |
| |
|
| Platform ID |
GPL3535 |
| Series (1) |
| GSE99800 |
Effects of Injectable DMPA and Combined Oral Contraceptive Use on HIV Susceptibility Factors in Pig-tailed Macaques |
|
