|
| Status |
Public on Jun 13, 2018 |
| Title |
siD1-3 |
| Sample type |
SRA |
| |
|
| Source name |
mesangial cell
|
| Organism |
Rattus |
| Characteristics |
strain: Wistar
passage: 10~15
genotype: DBP knock-down
cell type: mesangial cells
|
| Treatment protocol |
siRNA was transfected to inhibit the expression of DBP and NC siRNA was applied as control
|
| Growth protocol |
mesangial cells were cultured in 15% FBS 1640
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol
RNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Description |
Barcode: GTTTCGG
|
| Data processing |
The RAW-data was processed to map to Rattus norvegicus assembly 5.0.75 by Tophat v2.0.8
The data was dealed by cuffldiff v2.0.2 to calculate Fragments Kilobase of exon model per millon mapped reads (FPKM)
Supplementary_files_format_and_content: FPKMs
|
| |
|
| Submission date |
Jun 13, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Yang Lu |
| E-mail(s) |
lvyang1978@hotmail.com
|
| Organization name |
PLA general hospital
|
| Street address |
8th fuxing road
|
| City |
Beijing |
| ZIP/Postal code |
100853 |
| Country |
China |
| |
|
| Platform ID |
GPL23576 |
| Series (1) |
| GSE100001 |
mRNA expression profiling of siDBP-mesangial cells |
|
| Relations |
| BioSample |
SAMN07229709 |
| SRA |
SRX2915933 |
