|
| Status |
Public on Sep 29, 2008 |
| Title |
Construct Q74 Line 4 5 days (Q74_5_4_2) |
| Sample type |
RNA |
| |
|
| Source name |
PC12 cell culture
|
| Organism |
Rattus norvegicus |
| Characteristics |
Construct: Q74, Line 4
Time after induction: 5 days
|
| Treatment protocol |
Cells were treated with 1ug /ml doxycylin for the indicated number of days
|
| Growth protocol |
RNA was isolated from one 9 cm petriddish
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated with the RNeasy kit
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
Gene expression data from PC12 cells stably transfected with a doxycylin-inducible exon1 huntingtin fragment with normal-sized or elongated glutamine stretch
|
| Data processing |
Non-background corrected average intensities across all replicate beads (obtained from Beadstudio) were normalized with vsn
|
| |
|
| Submission date |
Feb 20, 2008 |
| Last update date |
Sep 29, 2008 |
| Contact name |
Peter A.C. 't Hoen |
| E-mail(s) |
p.a.c.hoen@lumc.nl
|
| Phone |
+31 71 5269421
|
| Fax |
+31 71 5268285
|
| URL |
http://www.humgen.nl
|
| Organization name |
Leiden University Medical Center
|
| Department |
Center for Human and Clinical Genetics
|
| Street address |
PO Box 9600
|
| City |
Leiden |
| ZIP/Postal code |
2300 RC |
| Country |
Netherlands |
| |
|
| Platform ID |
GPL6101 |
| Series (1) |
| GSE10581 |
Nrf2-related oxidative stress response and impaired dopamine biosynthesis in a PC12 cell model of Huntington’s disease |
|
