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| Status |
Public on Oct 21, 2017 |
| Title |
GH146-GFP [IL7051-704-502] |
| Sample type |
SRA |
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| Source name |
olfactory projection neuron
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| Organism |
Drosophila melanogaster |
| Characteristics |
genotype: GH146-GFP
cell type: olfactory projection neuron
number of cells: 1
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| Extracted molecule |
total RNA |
| Extraction protocol |
Modified SMART-seq2 (Li et al. 2017)
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| Library strategy |
OTHER |
| Library source |
transcriptomic |
| Library selection |
other |
| Instrument model |
NextSeq 550 |
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| Description |
Single cell
processed data files: df_libs.csv, htseq.tab, STAR.tab
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| Data processing |
Library strategy: 5’ RACE RNA-seq
alignment (STAR 2.4.2a, ENCODE standard options, except --outFilterScoreMinOverLread 0.4 --outFilterMatchNminOverLread 0.4 -- outFilterMismatchNmax 999 --outFilterMismatchNoverLmax 0.04)
gene count (HTSeq-count 0.7.1, default options, except -m intersection-strict)
Genome_build: Dmel r6.10
Supplementary_files_format_and_content: htseq.tab (gene counts), STAR.tab (summary of mapping results), df_libs.csv (sample information)
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| Submission date |
Jun 15, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Felix Horns |
| E-mail(s) |
rfhorns@stanford.edu
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| Organization name |
Stanford University
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| Department |
Bioengineering
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| Lab |
Quake
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| Street address |
Clark Center E300, Quake Lab
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| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305 |
| Country |
USA |
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| Platform ID |
GPL22106 |
| Series (1) |
| GSE100058 |
Classifying Drosophila Olfactory Projection Neuron Subtypes by Single-cell RNA Sequencing |
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| Relations |
| BioSample |
SAMN07245021 |
| SRA |
SRX2920154 |
| Supplementary data files not provided |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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