|
| Status |
Public on Jul 17, 2004 |
| Title |
+Gly20 (203587GLY20) |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Time course t=0 sample of strain W3031A grown on minimal
|
| Organism |
Saccharomyces cerevisiae |
| Extracted molecule |
total RNA |
| |
|
| Channel 2 |
| Source name |
Time course 20 min sample of strain W3031A after addition of 10 mM glycine
|
| Organism |
Saccharomyces cerevisiae |
| Extracted molecule |
total RNA |
| |
|
| |
| Description |
Comparison of cDNA made from mRNA of deletion set strain W3031A grown on minimal medium lacking glycine to cDNA made from mRNA from the same strain harvested 20 min after addition of 10 mM glycine.
The array slides were scanned with GenePix 4000B (Axon Instruments). Spots were found and analyzed with Quantarray 1.0 software (GSI Lumonics). Expression ratios were normalized and median centered for the Quantarray data files by Quantarray Data Handler 3.0. This software also applied filters for background and spot similarity so that low intensity spots at or near background levels and spots that differed more than 2-fold in their expression ratio were eliminated. Spot intensity and background data came from the Quantarray data files and the ratios of intensities at the two wavelengths were from the Quantarray Data Handler. The latter data were background subtracted normalized and averaged. Data points that are blank were removed by the background and spot similarity filters.
|
| |
|
| Submission date |
Jul 09, 2004 |
| Last update date |
May 27, 2005 |
| Contact name |
Andy L Bognar |
| E-mail(s) |
a.bognar@utoronto.ca
|
| Phone |
(416) 978-5854
|
| URL |
http://lambda.med.utoronto.ca/Bognar/
|
| Organization name |
University of Toronto
|
| Department |
Medical Genetics and Biology
|
| Lab |
Bognar Lab
|
| Street address |
1 King's College Circle
|
| City |
Toronto |
| State/province |
Ontario |
| ZIP/Postal code |
M5S 1A8 |
| Country |
Canada |
| |
|
| Platform ID |
GPL1337 |
| Series (1) |
| GSE1554 |
Time course of glycine addition or withdrawal |
|
