|
| Status |
Public on Feb 25, 2008 |
| Title |
Control leaves vs Stressed leaves_7d_rep2_A |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
maize DH4866 leaves
|
| Organism |
Zea mays |
| Characteristics |
maize DH4866 leaves
Age: at the heading stage
Tissue: maize flag leaves
|
| Treatment protocol |
The control plants were fully watered.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated as McCarty (1986) described.
|
| Label |
Cy5
|
| Label protocol |
Using SuperScript™ Indirect cDNA Labeling System (L1014-02)
|
| |
|
| Channel 2 |
| Source name |
maize DH4866 leaves
|
| Organism |
Zea mays |
| Characteristics |
maize DH4866 leaves
Age: at the heading stage
Tissue: maize flag leaves
|
| Treatment protocol |
The plants were stressed for 168 h (7 days) by watering every day to maintain a similar water-dificency state by adjusting water supply, according to the osmotic potential of the fully expended leaf of the plants measured at 8:00 am each day.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated as McCarty (1986) described.
|
| Label |
cy3
|
| Label protocol |
Using SuperScript™ Indirect cDNA Labeling System (L1014-02)
|
| |
|
| |
| Hybridization protocol |
according to the protocols at the website of the Maize Oligonucleotide Array Project.
|
| Scan protocol |
Scanned on an GenePix 4000B scanner.
Images were quantified using GENEPIX 6.0 software.
|
| Description |
na
|
| Data processing |
Global normalized, background subtracted data. VALUE column represents normalized, log2(test/reference) ratios.
|
| |
|
| Submission date |
Feb 21, 2008 |
| Last update date |
Feb 25, 2008 |
| Contact name |
juren zhang |
| E-mail(s) |
jrzhang@sdu.edu.cn
|
| Organization name |
shandong university
|
| Street address |
shanda nanlv 27
|
| City |
jinan |
| ZIP/Postal code |
250100 |
| Country |
China |
| |
|
| Platform ID |
GPL5439 |
| Series (1) |
| GSE10596 |
Differential gene expression analysis of maize leaf at heading stage in response to water-deficit stress |
|
