|
Status |
Public on Dec 31, 2017 |
Title |
COUP-KO 1 RA G+/M+ |
Sample type |
RNA |
|
|
Source name |
Human embryonic stem cells
|
Organism |
Homo sapiens |
Characteristics |
genotype: COUP-TFII mCherry/- tissue: Human embryonic stem cells treatment: Retinoic acid timepoint: week 3 of differentiation
|
Treatment protocol |
To direct differentiation to the atrial lineage cells were treated with 1 uM Retinoic acid (Sigma) from day 4 - 7 of differentiation. Cells were sorted for GFP and mCherry.
|
Growth protocol |
Differentiation of COUP-TFIImCherry/+-NKX2.5eGFP/+, COUP-TFIImCherry/--NKX2.5eGFP/+ and NKX2.5eGFP/+ hESCs to cardiomyocytes using the Spin-Embryoid body method.
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted using Macherey-Nagel Nucleospin Kit.
|
Label |
biotin
|
Label protocol |
Biotin-labeled (Biotin-16-UTP) amplified cRNA was generated using Illumina Totalprep-96 RNA Amplification Kit
|
|
|
Hybridization protocol |
Standard Illumina protocol: Whole-Genome Gene Expression Direct Hybridization Assay
|
Scan protocol |
Samples were scanned with illumina iscan array scanner using standard Illumina protocol
|
Description |
SAMPLE 4
|
Data processing |
The data was normalized using quantile normalization in Genespring GX v12.5
|
|
|
Submission date |
Jun 19, 2017 |
Last update date |
Dec 31, 2017 |
Contact name |
Robert Passier |
E-mail(s) |
robert.passier@utwente.nl
|
Organization name |
University of Twente
|
Department |
MIRA Institute for Biomedical Technology and Technical Medicine
|
Street address |
Building Zuidhorst
|
City |
Enschede |
ZIP/Postal code |
7500AE |
Country |
Netherlands |
|
|
Platform ID |
GPL10558 |
Series (1) |
GSE100199 |
A COUP-TFII human embryonic stem cell reporter line to identify and purify atrial cardiomyocytes |
|