|
| Status |
Public on Dec 31, 2017 |
| Title |
WT NKX-GFP RA G+ |
| Sample type |
RNA |
| |
|
| Source name |
Human embryonic stem cells
|
| Organism |
Homo sapiens |
| Characteristics |
genotype: WT +/+
tissue: Human embryonic stem cells
treatment: Retinoic acid
timepoint: week 3 of differentiation
|
| Treatment protocol |
To direct differentiation to the atrial lineage cells were treated with 1 uM Retinoic acid (Sigma) from day 4 - 7 of differentiation. Cells were sorted for GFP and mCherry.
|
| Growth protocol |
Differentiation of COUP-TFIImCherry/+-NKX2.5eGFP/+, COUP-TFIImCherry/--NKX2.5eGFP/+ and NKX2.5eGFP/+ hESCs to cardiomyocytes using the Spin-Embryoid body method.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using Macherey-Nagel Nucleospin Kit.
|
| Label |
biotin
|
| Label protocol |
Biotin-labeled (Biotin-16-UTP) amplified cRNA was generated using Illumina Totalprep-96 RNA Amplification Kit
|
| |
|
| Hybridization protocol |
Standard Illumina protocol: Whole-Genome Gene Expression Direct Hybridization Assay
|
| Scan protocol |
Samples were scanned with illumina iscan array scanner using standard Illumina protocol
|
| Description |
SAMPLE 6
|
| Data processing |
The data was normalized using quantile normalization in Genespring GX v12.5
|
| |
|
| Submission date |
Jun 19, 2017 |
| Last update date |
Dec 31, 2017 |
| Contact name |
Robert Passier |
| E-mail(s) |
robert.passier@utwente.nl
|
| Organization name |
University of Twente
|
| Department |
MIRA Institute for Biomedical Technology and Technical Medicine
|
| Street address |
Building Zuidhorst
|
| City |
Enschede |
| ZIP/Postal code |
7500AE |
| Country |
Netherlands |
| |
|
| Platform ID |
GPL10558 |
| Series (1) |
| GSE100199 |
A COUP-TFII human embryonic stem cell reporter line to identify and purify atrial cardiomyocytes |
|
