|
| Status |
Public on Jul 25, 2008 |
| Title |
Rhodopseudomonas palustris cells grown in the absence of pC-HSL #2 |
| Sample type |
RNA |
| |
|
| Source name |
Photosynthetic bacterium Rhodopseudomonas palustris
|
| Organism |
Rhodopseudomonas palustris CGA009 |
| Characteristics |
Rhodopseudomonas palustris cells grown in the absence of pC-HSL #2
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Rhodopseudomonas palustris cells were disrupted by bead beating and RNA was purified with the RNeasy Mini kit (Qiagen) including DNase treatment on the column.
|
| Label |
Biotinylated GeneChip reagent
|
| Label protocol |
As recommended by the manufacturer (Affymetrix)
|
| |
|
| Hybridization protocol |
As recommended by the manufacturer (Affymetrix)
|
| Scan protocol |
As recommended by the manufacturer (Affymetrix)
|
| Description |
A high-density oligonucleotide microarray (Affymetrix GeneChip) was custom designed and manufactured by Affymetrix based on the sequences of ORFs and intergenic regions. cDNA synthesis, fragmentation, labeling, hybridization, and processing of custom designed Rhodopseudomonas palustris GeneChips were carried out as recommended by the manufacturer. Experiments were performed in duplicate with independently prepared samples.
|
| Data processing |
The Affymetrix GeneChip Operating Software Version 1.4 was used for initial data acquisition and processing.
|
| |
|
| Submission date |
Feb 26, 2008 |
| Last update date |
Jul 25, 2008 |
| Contact name |
Yasuhiro Oda |
| E-mail(s) |
yasuhiro@uw.edu
|
| Organization name |
University of Washington
|
| Department |
Department of Microbiology
|
| Lab |
Harwood
|
| Street address |
Box 357242, 1705 N.E. Pacific Street, HSB K-354
|
| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98195-7242 |
| Country |
USA |
| |
|
| Platform ID |
GPL3954 |
| Series (1) |
| GSE10642 |
A New Class of Homoserine Lactone Quorum Sensing Signals |
|
