|
| Status |
Public on Dec 29, 2017 |
| Title |
BC sample: TM-012-10R |
| Sample type |
RNA |
| |
|
| Source name |
breast tumor tissue
|
| Organism |
Homo sapiens |
| Characteristics |
histology: IDC
grade: grade 2
gender: F
age (years): 48Y
her2 ihc status: HER2 1+
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA from biospecimens was extracted using the Quagen RNeasy mini kit according to the manufacturer's instructions and integrity of RNA was checked by using the Agilent Bioanalyzer.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNAs were prepared according to the standard Affymetrix protocol from 250 ng total RNA
|
| |
|
| Hybridization protocol |
Following fragmentation, 6 ug of cDNA were hybridized for 17 hr at 45C on GeneChip HuGene-1.0-st arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
|
| Description |
TM-012-10R
|
| Data processing |
Data were generated using the Affymetrix Expression Console Software using Affymetrix default analysis settings.
|
| |
|
| Submission date |
Jun 27, 2017 |
| Last update date |
Jan 23, 2018 |
| Contact name |
Hans-Juergen Schulten |
| E-mail(s) |
hschulten@kau.edu.sa
|
| Phone |
+966534355430
|
| Organization name |
King Abdulaziz University
|
| Department |
Center of Excellence in Genomic Medicine Research
|
| Lab |
Affymetrix Service Unit
|
| Street address |
P.O. Box 80216
|
| City |
Jeddah |
| ZIP/Postal code |
21589 |
| Country |
Saudi Arabia |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE100534 |
Expression profiling in breast cancer brain metastases compared to breast cancers and primary brain tumors |
|
| Relations |
| Alternative to |
GSM886566 |
