|
| Status |
Public on Sep 22, 2017 |
| Title |
Pml KO_apap2h_2 |
| Sample type |
RNA |
| |
|
| Source name |
Pml KO liver, 2h after apap ip
|
| Organism |
Mus musculus |
| Characteristics |
gender: female
tissue: Liver
age (months): 2.5
strain: BALB/cByJ
|
| Treatment protocol |
The mice were i.p. injected with Acetaminophen (Sigma) 300mg/kg solved in warmed PBS. They were sacrificed by cervical dislocation at indicated time. Untreated mice were sacrificed as a control. Liver samples are collected and stabilized in RNAlater solution and kept in -20. .
|
| Growth protocol |
Pml wt and Pml KO mice with BALB/cByJ mice are maintained in a 12-hour light/dark cycle animal facility under SPF conditions, with free access to water and food.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
total RNA were extracted from using miRNeasy kit (Qiagen)
|
| Label |
biotin
|
| Label protocol |
100ng of total RNA were amplified to generate cRNA; 10µg of cRNA were converted into single stranded DNA, based on the WT cDNA Synthesis Kit (Affymetrix). 5,5µg of cDNA were fragmented and labeled with the WT Terminal Labeling Kit (Affymetrix).
|
| |
|
| Hybridization protocol |
Samples were hybridized onto GeneChip Mouse Gene 1.1 ST Arrays (Affymetrix) using the GeneTitan platform at the Genomics platform, Translational Resarch Dept, Institut Curie , Paris, France. 3.3µg of targets were hybridized according Affymetrix recommendations.
|
| Scan protocol |
Array scanning was performed according to the manufacturer's instruction (Affymetrix)
|
| Description |
gene expression data from mouse liver sample using Affymetrix Mouse Gene 1.1 ST
|
| Data processing |
Raw data were processed with Expression Console from Affymetrix. A RMA normlisation was applied to control to data.
|
| |
|
| Submission date |
Jun 28, 2017 |
| Last update date |
Jan 23, 2018 |
| Contact name |
Michiko Kawakita |
| E-mail(s) |
rinko.kawa@gmail.com
|
| Organization name |
INSERM
|
| Department |
U944
|
| Street address |
1 av. Claude-Vellefaux
|
| City |
Paris |
| ZIP/Postal code |
75010 |
| Country |
France |
| |
|
| Platform ID |
GPL11533 |
| Series (2) |
| GSE100616 |
Gene expression profiling of Pml wt and Pml KO mice liver with acetaminophen (apap) overdose (300mg/kg) i.p. |
| GSE100617 |
PML is a ROS sensor activating p53 upon oxidative stress |
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