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Status |
Public on Jun 30, 2018 |
Title |
42W-1 |
Sample type |
genomic |
|
|
Source name |
42W-1
|
Organism |
Mus musculus |
Characteristics |
tissue: Blood disease: T2D
|
Treatment protocol |
Genomic DNA was extracted from peripheral blood leukocytes by using the Genomic DNA extraction kit (Qiagen, Valencia, CA, USA), according to the manufacturer’s instructions.
|
Growth protocol |
Peripheral blood leukocytes was collected from T2D mice and control mice.
|
Extracted molecule |
genomic DNA |
Extraction protocol |
One microgram of genomic DNA was sonicated to produce fragments ranging from 100 to 500 bp.
|
Label |
biotin
|
Label protocol |
Then, 3.5 mg (7 mL) of MBD-Biotin Protein was added to 10 mL Dynabeads M-280 Streptavidin according to the manufacturer’s instructions.
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|
|
Hybridization protocol |
Hybridization was performed with an Affymetrix GeneChip Promoter 1.0R array.
|
Scan protocol |
Array was scanned on an Affymetrix GeneChip Scanner 3000.
|
Description |
fraction: promoter
|
Data processing |
Hybridization was performed with an Affymetrix GeneChip Promoter 1.0R array. The resulting processed data are generated by the Affymetrix Tiling Analysis Software.
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|
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Submission date |
Jun 30, 2017 |
Last update date |
Jun 30, 2018 |
Contact name |
Yng-Tay Chen |
E-mail(s) |
t10002@mail.cmuh.org.tw
|
Organization name |
China Medical University Hospital
|
Department |
Department of medical research
|
Lab |
Human Genetic center
|
Street address |
No 2 Yude road
|
City |
Taichung |
ZIP/Postal code |
40447 |
Country |
Taiwan |
|
|
Platform ID |
GPL5811 |
Series (1) |
GSE100677 |
DNA methylation profiling of promoter DNA associated to the T2D. |
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