|
| Status |
Public on Jun 30, 2018 |
| Title |
K02 |
| Sample type |
genomic |
| |
|
| Source name |
K02
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Blood
disease: Normal
|
| Treatment protocol |
Genomic DNA was extracted from peripheral blood leukocytes by using the Genomic DNA extraction kit (Qiagen, Valencia, CA, USA), according to the manufacturer’s instructions.
|
| Growth protocol |
Peripheral blood leukocytes was collected from T2D mice and control mice.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
One microgram of genomic DNA was sonicated to produce fragments ranging from 100 to 500 bp.
|
| Label |
biotin
|
| Label protocol |
Then, 3.5 mg (7 mL) of MBD-Biotin Protein was added to 10 mL Dynabeads M-280 Streptavidin according to the manufacturer’s instructions.
|
| |
|
| Hybridization protocol |
Hybridization was performed with an Affymetrix GeneChip Promoter 1.0R array.
|
| Scan protocol |
Array was scanned on an Affymetrix GeneChip Scanner 3000.
|
| Description |
fraction: promoter
|
| Data processing |
Hybridization was performed with an Affymetrix GeneChip Promoter 1.0R array.
The resulting processed data are generated by the Affymetrix Tiling Analysis Software.
|
| |
|
| Submission date |
Jun 30, 2017 |
| Last update date |
Jun 30, 2018 |
| Contact name |
Yng-Tay Chen |
| E-mail(s) |
t10002@mail.cmuh.org.tw
|
| Organization name |
China Medical University Hospital
|
| Department |
Department of medical research
|
| Lab |
Human Genetic center
|
| Street address |
No 2 Yude road
|
| City |
Taichung |
| ZIP/Postal code |
40447 |
| Country |
Taiwan |
| |
|
| Platform ID |
GPL5811 |
| Series (1) |
| GSE100677 |
DNA methylation profiling of promoter DNA associated to the T2D. |
|
