|
| Status |
Public on Jun 06, 2020 |
| Title |
infantile hemangioama tumor tissue microRNA 3 |
| Sample type |
RNA |
| |
|
| Source name |
infantile hemangioma (IH) tumor tissue
|
| Organism |
Homo sapiens |
| Characteristics |
age: 6 months
gender: male
|
| Treatment protocol |
Tissues from IH surgury were immersed overnight in RNAlater at 4 ℃ and preserved at -80 ℃.
|
| Growth protocol |
IH and matched non-tumor skin tissues
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix)
|
| |
|
| Hybridization protocol |
Following fragmentation, cRNA were hybridized for 16 hr at 48C on GeneChip Hybridization Oven 645. GeneChips were washed and stained in the Affymetrix Fluidics Station 450
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000 7G.
|
| Description |
microRNA expression data from infantile hemangioma (IH) tumor tissue
|
| Data processing |
The data were analyzed with RMA using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
Jun 30, 2017 |
| Last update date |
Jun 06, 2020 |
| Contact name |
Cong Fu |
| E-mail(s) |
cong3399@outlook.com
|
| Organization name |
Shandong provincial hospital
|
| Street address |
No. 324, jingwu Road, Huaiyin Dist
|
| City |
Jinan |
| State/province |
Shandong |
| ZIP/Postal code |
250021 |
| Country |
China |
| |
|
| Platform ID |
GPL21572 |
| Series (1) |
| GSE100682 |
microRNA expression in infantile hemangioma (IH) and normal skin tissues |
|
