|
| Status |
Public on May 29, 2019 |
| Title |
Nfkbiz KO keratinocyte v-rasHa transduced, biological rep 3 |
| Sample type |
RNA |
| |
|
| Source name |
KO RAS
|
| Organism |
Mus musculus |
| Characteristics |
genoptype: Nfkbiz KO v-rasHa transduced
strain: C57BL/6
cell type: keratinocyte
|
| Treatment protocol |
v-rasHa transduction for 3 days
|
| Growth protocol |
Keratinocyte grown in regular media (no starvation)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol + Qiagen column purification
|
| Label |
biotin
|
| Label protocol |
procedure performed by Genomics Laboratory Cancer Research Technology Program Frederick National Laboratory for Cancer Research http://atp.ncifcrf.gov
|
| |
|
| Hybridization protocol |
procedure performed by Genomics Laboratory Cancer Research Technology Program Frederick National Laboratory for Cancer Research http://atp.ncifcrf.gov
|
| Scan protocol |
procedure performed by Genomics Laboratory Cancer Research Technology Program Frederick National Laboratory for Cancer Research http://atp.ncifcrf.gov
|
| Data processing |
Raw gene expression data (.CEL files) were processed using Qlucore Omics Explorer (QOE) software (version 3; Qlucore AB, Lund, Sweden).
|
| |
|
| Submission date |
Jul 05, 2017 |
| Last update date |
May 29, 2019 |
| Contact name |
Christophe Cataisson |
| Organization name |
National Cancer Institute
|
| Lab |
Laboratory of Cancer Biology and Genetics
|
| Street address |
37 Covent drive
|
| City |
Bethesda |
| State/province |
Maryland |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE100836 |
Effect of IkBz (Nfkbiz) deficiency on oncogenic RAS transcriptional program |
|
