NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM2695305 Query DataSets for GSM2695305
Status Public on Jul 31, 2017
Title H1299-DMSO
Sample type RNA
 
Source name DMSO treated H1299 cells
Organism Homo sapiens
Characteristics cell line: H1299
Treatment protocol H1299 cells were treated with either 20µM PD98059 (MEK inhibitor) or DMSO for 2 days
Growth protocol Vector control and KRAS-G12V transfected HBEC3 cells were cultured in keratinocyte serum-free media supplemented with bovine pituitary extract and recombinant human EGF. H1299 cells were cultured in RPMI-1640 media supplemented with 10% FBS.
Extracted molecule total RNA
Extraction protocol RNA was isolated using the mirVANA miRNA isolation kit (Ambion, LifeTechnologies) as per manufacturer instructions.
Label biotin
Label protocol Samples were given to Genomics Division of the Iowa Institute of Human Genetics for miRNA profiling. Affymetrix GeneChip™ miRNA 4.0 Assay was utilized by the facility.
 
Hybridization protocol As per Affymetrix GeneChip™ miRNA 4.0 Assay
Scan protocol Affymetrix GeneChip Model 3000 GeneChip Scanner was used for scanning
Description miRNA profiling data from DMSO treated H1299 cells
Data processing Data was processed using Affymetrix Expression Console and Transcriptome Analysis Console software 3.0.
 
Submission date Jul 06, 2017
Last update date Jan 23, 2018
Contact name Charles Brenner
Organization name University of Iowa
Department Biochemistry, Carver College of Medicine
Lab 4-339
Street address Bowen Science Building, Newton Road
City Iowa
ZIP/Postal code 52242
Country USA
 
Platform ID GPL21572
Series (1)
GSE100857 KRAS-driven miR-29b Expression is Required for Tumor Suppressor Gene Silencing

Data table header descriptions
ID_REF
VALUE Quantification
DETECTION P-VALUE

Data table
ID_REF VALUE DETECTION P-VALUE
20500112 10.369 8.72176e-16
20500113 1.00343 0.918918
20500114 3.30594 4.65824e-05
20500115 8.44674 8.72176e-16
20500116 1.58708 0.0185505
20500117 6.89047 2.25824e-15
20500118 0.911412 0.894358
20500119 6.7558 8.72176e-16
20500120 1.28697 0.142956
20500121 9.79066 8.72176e-16
20500122 2.02847 0.00113789
20500123 4.457 2.0016e-10
20500124 1.26963 0.305961
20500125 1.02455 0.842932
20500126 7.71742 8.72176e-16
20500127 0.962053 0.441238
20500128 12.6095 8.72176e-16
20500129 1.15167 0.676995
20500130 12.4227 8.72176e-16
20500131 3.68139 6.25844e-07

Total number of rows: 6658

Table truncated, full table size 171 Kbytes.




Supplementary file Size Download File type/resource
GSM2695305_H1299-DMSO.CEL.gz 733.6 Kb (ftp)(http) CEL
GSM2695305_H1299-DMSO.chp.gz 66.1 Kb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap