|
| Status |
Public on Dec 31, 2017 |
| Title |
Young adult keratinocytes- repl8 |
| Sample type |
RNA |
| |
|
| Source name |
Young adult keratinocytes
|
| Organism |
Homo sapiens |
| Characteristics |
age: 20-40 years
cell type: Human primary keratinocytes
passage: ealy passage cells (P2)
|
| Growth protocol |
Human primary keratinocytes were cultured in KGM2 medium and RNA was extracted in ealy passage cells (P2) at confluency
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Extraction of total RNA including small RNA was performed using the miRvana isolation kit according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standart Affymetrix protocol from 1 ug of total RNA
|
| |
|
| Hybridization protocol |
Following fragmentation, 1 ug of cRNA washybridized for 16 hr at 45C on GenChip miRNA 4.0
|
| Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000 7G
|
| Description |
JL8
|
| Data processing |
Scanned images of microarray chips were analyzed using Expression Console software (Affymetrix) with the default settings.
affymetrix-algorithm-name = rma : dabg
affymetrix-algorithm-version = 1.0
affymetrix-array-type = miRNA-4_0
program-name = Expression Console
program-version = 1.3.1.187
|
| |
|
| Submission date |
Jul 17, 2017 |
| Last update date |
Jan 23, 2018 |
| Contact name |
Jerome Lamartine |
| E-mail(s) |
jerome.lamartine@univ-lyon1.fr
|
| Phone |
+33 4 72 72 26 66
|
| Organization name |
Université Lyon I
|
| Lab |
LBTI
|
| Street address |
7 passage du Vercors
|
| City |
LYON |
| ZIP/Postal code |
F69367 |
| Country |
France |
| |
|
| Platform ID |
GPL21572 |
| Series (1) |
| GSE101493 |
microRNA expression in human keratinocytes from infant, adult and ederly skin |
|
