|
| Status |
Public on Jul 19, 2017 |
| Title |
5_NOD-2 |
| Sample type |
genomic |
| |
|
| Source name |
male NOD mice 12 weeks old
|
| Organism |
Mus musculus |
| Characteristics |
strain: NOD
Sex: male
age: 12 weeks old
|
| Treatment protocol |
No treatment of mice before DNA extraction
|
| Growth protocol |
All mice were maintained under specific pathogen-free conditions and experimental studies were performed in accordance with the Institutional Animal Care and Use Guidelines and accredited by the ethic committee under the number CEEA34.CB.024.11.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
DNA extraction were realized from tails by 20 mg/ml proteinase K (Invitrogen) degradation, saturated NaCl/propanol DNA precipitation and DNA re-suspension 2 hours in TE10-4 buffer at 42°C.
|
| Label |
Biotin
|
| Label protocol |
DNA was restriction digested, PCR amplified, fragmented and labeled according to the manufacturer's instructions using Genome-Wide SNP6.0 Assay kit.
|
| |
|
| Hybridization protocol |
DNA was hybridized to each array according to the manufacturer's instructions using Genome-Wide SNP 6.0 Assaykit.
|
| Scan protocol |
The Arrays were then washed using Affymetrix fluidics stations, and scanned using the Gene Chip Scanner 3000.
|
| Data processing |
The array image was acquired using Affymetrix Genotyping Console (version 4.2.0.26). SNP calls were extracted and converted from CEL files into signal intensities using Genotyping Console (version 4.2.0.26) software and BRLMM algorithm.
|
| |
|
| Submission date |
Jul 18, 2017 |
| Last update date |
Jan 23, 2018 |
| Contact name |
Franck Letourneur |
| E-mail(s) |
franck.letourneur@inserm.fr
|
| Organization name |
INSERM
|
| Department |
U1016
|
| Lab |
genomic
|
| Street address |
22 rue mechain
|
| City |
Paris |
| ZIP/Postal code |
75014 |
| Country |
France |
| |
|
| Platform ID |
GPL13147 |
| Series (1) |
| GSE101551 |
A humanized mouse model to study type 1 diabetes |
|
