|
| Status |
Public on Mar 05, 2008 |
| Title |
transduced with eGFP (1132) |
| Sample type |
RNA |
| |
|
| Source name |
transduced with eGFP
|
| Organism |
Homo sapiens |
| Characteristics |
human umbilical vein endotelial cells (HUVEC)
cultured on plastic in 2D, transduced with eGFP
|
| Treatment protocol |
Cultured cells were overlayed with approximately 10x their mass of Trizol then disrupted with a cell scraper.
|
| Growth protocol |
Genetically-modified HUVEC grown on a variety of substrates and in a variety of geometries were comared.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNAs were prepared from 5 micrograms total RNA according to the standard Affymetrix protocol.
|
| |
|
| Hybridization protocol |
After fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45 dgrees C to the geneChips, which were then washed and stained according to standard Affymetrix protocols.
|
| Scan protocol |
Scanned with GeneArray Scanner G2500A.
|
| Description |
Gene expression data from HUVEC cultured in different conditions or differently genetically modified
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100. The data were then normalised by chips using he loess method in R.
|
| |
|
| Submission date |
Mar 04, 2008 |
| Last update date |
Mar 16, 2009 |
| Contact name |
Cris Print |
| E-mail(s) |
c.print@auckland.ac.nz
|
| Organization name |
Auckland University
|
| Street address |
Grafton Campus, Park Rd
|
| City |
Auckland |
| ZIP/Postal code |
1 |
| Country |
New Zealand |
| |
|
| Platform ID |
GPL8300 |
| Series (1) |
| GSE10710 |
Bcl2-induced vascular maturation |
|
