|
| Status |
Public on Jan 29, 2009 |
| Title |
vCRCCD09 |
| Sample type |
RNA |
| |
|
| Source name |
human peripheral blood sample
|
| Organism |
Homo sapiens |
| Characteristics |
Blood: CRC stage Duke C, D
|
| Extracted molecule |
total RNA |
| Extraction protocol |
PaxGene extraction of total RNA was performed according to the manufacturer instructions
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 microg total RNA (Affymetrix Globin Reduction Protocol)
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on Affymetrix HGU133 Plus 2.0 chip. GeneChips were washed and stained in the Affymetrix Fluidics Station 450
|
| Scan protocol |
GeneChips were scanned using the GeneChip 3000 Scanner
|
| Description |
Gene expression data from peripheral blood sample
|
| Data processing |
The data were preprocessed by the function mas5 from the Bioconductor affy package
|
| |
|
| Submission date |
Mar 04, 2008 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Norbert Solymosi |
| E-mail(s) |
solymosi.norbert@gmail.com
|
| Phone |
36309347069
|
| Organization name |
ATE
|
| Street address |
István u. 2.
|
| City |
Budapest |
| State/province |
Budapest |
| ZIP/Postal code |
1078 |
| Country |
Hungary |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE10715 |
Expression data from normal and CRC human peripheral blood samples |
|
| Relations |
| Reanalyzed by |
GSE119087 |
