|
| Status |
Public on Oct 31, 2017 |
| Title |
6606 Sub LN 32hr |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Submandibular lymph node 32hr post-infection
|
| Organism |
Chlorocebus aethiops |
| Characteristics |
gender: Male
tissue: Lymph Nodes
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Trizol following manufacturer's instructions
|
| Label |
Cy5
|
| Label protocol |
Total RNA (200-2000ng) was used as starting material, which was amplified using the Quick Amp Two-Color Kit (Agilent Technologies).The labelled cDNA was purified using an RNeasy Mini Kit (Qiagen, Hilden, Germany), and eluted, labelled cRNA was quantified using a NanoDrop ND-1000 spectrophotometer (Thermo Fisher Scientific Inc, Waltham, MA, USA).
|
| |
|
| Channel 2 |
| Source name |
Monkey Universal RNA
|
| Organisms |
Macaca fascicularis; Macaca mulatta |
| Characteristics |
tissue: several major organs
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Trizol following manufacturer's instructions
|
| Label |
Cy3
|
| Label protocol |
Total RNA (200-2000ng) was used as starting material, which was amplified using the Quick Amp Two-Color Kit (Agilent Technologies).The labelled cDNA was purified using an RNeasy Mini Kit (Qiagen, Hilden, Germany), and eluted, labelled cRNA was quantified using a NanoDrop ND-1000 spectrophotometer (Thermo Fisher Scientific Inc, Waltham, MA, USA).
|
| |
|
| |
| Hybridization protocol |
cRNA was hybridized to a whole mouse genome microarray (Agilent Technologies) following manufacturer's instructions
|
| Scan protocol |
Scanned on a Agilent Technologies Scanner G2505C Images were quantified using Agilent Feature Extraction Software
|
| Description |
Submandibular lymph node 32hr
|
| Data processing |
Agilent Feature Extraction Software (v 10.7) was used for background subtraction and GeneSpring v.10.1 (Agilent Technologies, Inc., CA) software was used to conduct the preliminary data filtration and statistical analysis. Filtered data available on Series record supplementary file.
|
| |
|
| Submission date |
Jul 19, 2017 |
| Last update date |
Jan 23, 2018 |
| Contact name |
Aarti Gautam |
| E-mail(s) |
aarti.gautam.civ@health.mil
|
| Phone |
301-619-7683
|
| Organization name |
WRAIR
|
| Department |
Medical Readiness Systems Biology
|
| Street address |
503 Robert Grant Avenue
|
| City |
Silver Spring |
| State/province |
MD |
| ZIP/Postal code |
20910 |
| Country |
USA |
| |
|
| Platform ID |
GPL9861 |
| Series (1) |
| GSE101653 |
Submandibular lymph node, a key battleground for host-Yersinia pestis interaction: A transcriptomic study using non-human primate model |
|
