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| Status |
Public on Dec 22, 2017 |
| Title |
DL_HiC_E12_del1-13d9lac |
| Sample type |
SRA |
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| Source name |
Distal forelimbs and distal hindlimbs
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| Organism |
Mus musculus |
| Characteristics |
tissue: Distal forelimbs and distal hindlimbs
genotype: delHoxd(1-13)d9lac
strain: B6CBAF3
embryonic day: E12.5
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| Extracted molecule |
genomic DNA |
| Extraction protocol |
Hi-C libraries were generated using the HindIII enzyme as described in the protocol outlined in (Belton et al. 2012).
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| Library strategy |
Hi-C |
| Library source |
genomic |
| Library selection |
other |
| Instrument model |
Illumina HiSeq 4000 |
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| Description |
Distal limb mouse E12.5_del1-13d9lac_HiC
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| Data processing |
Reads were mapped, filtered and bias corrected as described before (Lajoie et al. Methods 2015, Giorgetti Nature 2016).
Briefly, read pairs were mapped independently, starting at 25bp and iterated every 5bp using bowtie2 (version 2.2.4 (Langmead et al. 2009)) (as described in Imakaev Nature methods 2012) with parameter: --very-sensitive, either on mouse genome (mm10), or on the mutant genomes generated in silico. Each read was assigned to a fragment using the 5’ mapped-position shifted 3 bp toward the 3’ position to assign correctly the reads overlapping the cutting sites. The fragment assignment and mapping strand from R1 and R2 was combined and used to filter out the single-side mapped pairs, dangling-end pairs, error pairs and self-circle pairs. For each condition (same tissue and same genotype), two replicates were merged (fifteen replicates for CH12) and the interactions were filtered to discard duplicates. Each fragment was assigned to a bin (40 or 20kb) based on the position of the middle of the fragment and each valid interaction was assigned to a couple pair of bins (one bin for the R1 and one bin for the R2) and reported in the raw matrix. Prior to the ICE normalization (Imakaev et al. 2012), the rows and columns were masked if the sum of reads in this region was 10 fold less the expectation with uniform coverage, or if the number of fragments covered by at least two reads in this region was less than half the number of the fragments of this region.
Genome_build: mm10 (for DL_Wt, PL_Wt, DL_delattP-Rel5d9lac, PL_delattP-Rel5d9lac, CH12 and mESC) and mutant genomes (for DL_delattP-Rel5d9lac, PL_delattP-Rel5d9lac, DL_del1-13d9lac and PL_del1-13d9lac which are available at DOI: 10.6084/m9.figshare.5220637)
Supplementary_files_format_and_content: validPair.txt.gz contains every valid pair (not asigned to the same fragments) for each dataset. Each line is a read, column 2 is the chromosome name of the first read, column 3 is the coordinate of this read, column 4 is the strand, column 5 is the read name, column 6 is the fragment number to which this read has been asigned, columns 8-12 are the same for the other read. This file is not filtered for duplicates. __txt.tar contains all cis matrices of interactions in one folder for each resolution (20kb or 40kb bin size) and then one subfolder for the raw and one subfolder for the iced (normalised) matrices. At the 40kb resolution, cis interactions for all chromosomes are provided. At 20kb resolution, only the matrices for chr2 are provided.
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| Submission date |
Jul 20, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Eddie Rodríguez-Carballo |
| E-mail(s) |
edgardo.rodriguez@unige.ch
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| Organization name |
Université de Genève
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| Department |
Department of Genetics and Evolution
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| Street address |
4, Boulevard d'Yvoy
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| City |
Geneva |
| ZIP/Postal code |
1205 |
| Country |
Switzerland |
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| Platform ID |
GPL21103 |
| Series (2) |
| GSE101715 |
A TAD boundary at the HoxD locus segregates opposing limb regulatory landscapes and their target genes [Hi-C] |
| GSE101717 |
A TAD boundary at the HoxD locus segregates opposing limb regulatory landscapes and their target genes |
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| Relations |
| BioSample |
SAMN07374541 |
| SRA |
SRX3024412 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSM2713718_DL_HiC_E12_del1-13d9lac.validPair.txt.gz |
1.5 Gb |
(ftp)(http) |
TXT |
| GSM2713718_DL_HiC_E12_del1-13d9lac_txt.tar.gz |
215.7 Mb |
(ftp)(http) |
TAR |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
| Processed data are available on Series record |
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